PNA-DNA hybridization at the air-water interface in the presence of octadecylamine langmuir monolayers

被引：16

作者：

Ramakrishnan, V ^{[1
]}

D'Costa, M ^{[1
]}

Ganesh, KN ^{[1
]}

Sastry, M ^{[1
]}

机构：

[1] Natl Chem Lab, Mat Chem & Organ Chem Synth Div, Pune 411008, Maharashtra, India

来源：

LANGMUIR | 2002年 / 18卷 / 16期

关键词：

D O I：

10.1021/la025755t

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Sequential immobilization of single-stranded complementary DNA and peptide nucleic acid (PNA) molecules at the air-water interface with cationic Langmuir monolayers is demonstrated. The complexation of the single-stranded DNA/PNA molecules with octadecylamine (ODA) Langmuir monolayers was followed in time by monitoring the pressure-area isotherms. A large (and slow) expansion of the ODA monolayer was observed during immobilization of single-stranded DNA at the air-water interface, and interestingly, no further expansion was detected on addition of PNA to the subphase. However, quartz crystal microgravimetry and UV-melting analysis of Langmuir-Blodgett films of the ODA-PNA-DNA hybrid monolayer clearly showed that the sequential immobilization process had resulted in hybridization of the complementary single-stranded DNA molecules with PNA at the air-water interface.

引用

页码：6307 / 6311

页数：5

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