Selection of methylation procedures for quantitation of short-chain glycerol-bound compounds formed during thermoxidation

被引：36

作者：

Berdeaux, O ^{[1
]}

Marquez-Ruiz, G ^{[1
]}

Dobarganes, C ^{[1
]}

机构：

[1] CSIC, Inst Grasa, Seville 41012, Spain

来源：

JOURNAL OF CHROMATOGRAPHY A | 1999年 / 863卷 / 02期

关键词：

methylation; fatty acids; aldehydic acids; acetals;

D O I：

10.1016/S0021-9673(99)00974-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Five methylation procedures, including base- and acid-catalyzed methods, were tested in thermoxidized methyl linoleate and trilinolein, in order to quantitate major oxidation short-chain glycerol-bound compounds by gas chromatography. Results indicated that transmethylations using KOH in methanol or CH3ONa-CH3OH in tert.-butylmethyl ether were the most appropriate methods, given the excellent reproducibility and practically complete recovery obtained for the compounds of interest, mainly short-chain fatty acids and aldehydic acids. Also, formation of acids from aldehydes during thermoxidation as well as modifications of aldehydic functions under acidic conditions, such as conversion to acetals, were checked using dodecanal as model aldehyde. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：171 / 181

页数：11

共 37 条

[11] DOBARGANES MC, 1986, GRASAS ACEITES, V37, P61
[12] ESTERBAUER H, 1991, FREE RADICAL BIO MED, V11, P841
[13] Frankel E.N., 1985, FLAVOR CHEM FATS OIL, P1
[14] Frankel E.N., 1998, Lipid oxidation
[15] The formation of new compounds
Gasparoli, A
[J]. GRASAS Y ACEITES, 1998, 49 (3-4) : 303 - 309
[16] Graille J., 1976, Annales des Falsifications et de l'Expertise Chimique, V69, P179
[17] *IUPAC, 1992, STAND METH ANAL O S1
[18] Characterisation of aldehydic acids in used and unused frying oils
KamalEldin, A
MarquezRuiz, G
Dobarganes, C
Appelqvist, LA
[J]. JOURNAL OF CHROMATOGRAPHY A, 1997, 776 (02) : 245 - 254
[19] Aldehydic acids in frying oils: Formation, toxicological significance and analysis.
KamalEldin, A
Appelqvist, LA
[J]. GRASAS Y ACEITES, 1996, 47 (05) : 342 - 348
[20] PREPARATION, CHROMATOGRAPHY AND MASS-SPECTROMETRY OF CHOLESTERYL ESTER AND GLYCEROLIPID-BOUND ALDEHYDES
KAMIDO, H
KUKSIS, A
MARAI, L
MYHER, JJ
PANG, H
[J]. LIPIDS, 1992, 27 (08) : 645 - 650

← 1 2 3 4 →