Transgenic rice plants conferring increased tolerance to rice blast and multiple environmental stresses

被引:15
|
作者
Uchimiya, H [1 ]
Fujii, S
Huang, JR
Fushimi, T
Nishioka, M
Kim, KM
Yamada, MK
Kurusu, T
Kuchitsu, K
Tagawa, M
机构
[1] Univ Tokyo, Inst Mol & Cellular Biosci, Bunkyou Ku, Tokyo 1130032, Japan
[2] Nissan Chem Ind Co Ltd, Shiraoka Res Stn Biol Sci, Minami Saitama, Saitama 3490294, Japan
[3] Tokyo Univ Sci, Dept Appl Biol Sci, Chiba 2788510, Japan
关键词
abiotic stresses; blast disease tolerance; NADPH dependent HC-toxin reductase; Oryza sativa;
D O I
10.1023/A:1019275218830
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
We isolated a rice gene (denoted YK1), which showed 78 percent amino acid sequence homology to the maize HM1 gene. A chimeric gene consisting of a promoter and first intron of maize ubiquitin gene and the cDNA of YK1 was introduced into rice via Agrobacterium mediated transformation. Transgenic rice plants overexpressing this chimeric gene were resistant to rice blast (Magnaporthe grisea) disease, which is one of the most serious pathogens in rice. Furthermore, the same transgenic plants conferred high tolerance to several abiotic stresses such as NaCl, UV-C, submergence, and hydrogen peroxide.
引用
收藏
页码:25 / 31
页数:7
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