Immunocytochemical localization of adrenomedullin 2/intermedin-like immunoreactivity in human hypothalamus, heart and kidney

被引：58
作者：
Takahashi, Kazuhiro
Kikuchi, Kumi
Maruyama, Yutaka
Urabe, Tomoko
Nakajima, Kiichiro
Sasano, Hironobu
Imai, Yutaka
Murakami, Osamu
Totsune, Kazuhito
机构：
[1] Tohoku Univ, Sch Hlth Sci, Dept Analyt Med Technol, Aoba Ku, Sendai, Miyagi 9808575, Japan
[2] Tohoku Univ, Sch Med, Dept Pathol, Aoba Ku, Sendai, Miyagi 9808575, Japan
[3] Tohoku Univ, Grad Sch Pharmaceut Sci & Med, Dept Clin Pharmacol & Therapeut, Aoba Ku, Sendai, Miyagi 9808575, Japan
[4] Tohoku Univ, 21st COE Program, Comprehens Res & Educ Ctr Planning Drug Dev & Cli, Aoba Ku, Sendai, Miyagi 9808575, Japan
[5] Peptide Inst Inc, Osaka 5628686, Japan
[6] Tohoku Univ Hosp, Dept Med, Aoba Ku, Sendai, Miyagi 9808574, Japan
来源：
PEPTIDES | 2006年 / 27卷 / 06期
关键词：
adrenomedullin; intermedin; immunocytochemistry; hypothalamus; heart; kidney;
D O I：
10.1016/j.peptides.2005.11.004
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Adrenomedullin 2/intermedin (AM2/IMD) is a novel member of the calcitonin/calcitonin gene-related peptide (CGRP) peptide family. AM2/IMD has a vasodilator action, and antidiuretic and antinatriuretic effects in mice. The aim of the present study is to clarify immunolocalization of AM2/IMD in human hypothalamus, heart and kidney obtained at autopsy. Immunocytochemistry showed AM2/IMD-immunoreactive cell bodies in the paraventricular and supraoptic nuclei of human hypothalamus. Both parvocellular and magnocellular cells in the paravetricular nucleus are immunostained with AM2/IMD. Immunostaining of serial sections showed co-localization of AM2/IMD-like immunoreactivity and vasopressin in the paraventricular nucleus. Myocardial cells of the heart and renal tubular cells were positively immunostained with AM2/IMD, whereas neither renal glomeruli nor vasculature in the heart and kidney were immunostained. Reverse-transcriptase polymerase chain reaction confirmed expression of AM2/IMD mRNA in the brain, pituitary, heart and kidney. The present study has shown the wide expression of AM2/IMD in human hypothalamus, heart and kidney, raising the possibility that this novel peptide may be related to the central and peripheral regulation of the circulation and water-electrolyte metabolism. (c) 2005 Elsevier Inc. All rights reserved.
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页码：1383 / 1389
页数：7
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