Detection of TT virus by single-primer sequence-independent amplification in multiple samples collected from an outbreak of gastroenteritis

被引：10
作者：
Braham, S. [1 ]
Iturriza-Gomara, M. [1 ]
Gray, J. [1 ]
机构：
[1] Hlth Protect Agcy, Ctr Infect, Enter Virus Unit, Virus Reference Dept, London NW9 5HT, England
来源：
ARCHIVES OF VIROLOGY | 2009年 / 154卷 / 06期
关键词：
TORQUE TENO VIRUS; INFECTIOUS INTESTINAL DISEASE; MOLECULAR-DETECTION; FECAL SAMPLES; NON-A; DNA; IDENTIFICATION; TRANSMISSION; CIRCOVIRUS; DISCOVERY;
D O I：
10.1007/s00705-009-0387-8
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A panel of gastroenteritis outbreak samples was subjected to a virus purification and concentration algorithm followed by a sequence-independent amplification method devised to detect viral enteric pathogens. The application of these methods allowed the identification of torque teno virus (TTV) in one outbreak. The complete genome sequence of 3,260 nt was obtained through "genome walking", and four open reading frames were deduced from the genomic sequence. Phylogenetic analysis grouped this virus in TTV genetic group 3, clustering with genotype 27, with 85% similarity at the nt level with strain SAa-01.
引用
收藏
页码：981 / 985
页数：5
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