Quantitative LC-MS/MS method for the sensitive and simultaneous determination of natural hormones in bovine serum

被引：42

作者：

Regal, P. ^{[1
]}

Vazquez, B. I. ^{[1
]}

Franco, C. M. ^{[1
]}

Cepeda, A. ^{[1
]}

Fente, C. ^{[1
]}

机构：

[1] Univ Santiago de Compostela, Fac Vet, Lugo 27002, Spain

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2009年 / 877卷 / 24期

关键词：

Hormone; LC-MS/MS; Bovine serum; TANDEM MASS-SPECTROMETRY; PERFORMANCE LIQUID-CHROMATOGRAPHY; ELECTROSPRAY-IONIZATION; STEROID-HORMONES; DERIVATIZATION; ESTROGENS; RESIDUES; TESTOSTERONE; PLASMA; ASSAY;

D O I：

10.1016/j.jchromb.2009.06.025

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a liquid chromatography tandem mass spectrometric (LC-MS/MS) method for the simultaneous quantitative analysis of several free forms of steroid hormones in bovine serum I pregnenolone (P-5), progesterone (P-4), 17hydroxyP(5), 17hydroxyP(4), testosterone (T), dehydroepiandrosterone (DHEA), androstenedione (A), estrone (E-1), 2,4 and 16 hydroxyE(1), 2 and 4 methoxyE(1)]. Deuterated analogs were used as internal standards. Serum proteins were eliminated with acetonitrile. Oxime derivatives of steroids were extracted with tert-butylmethylether and analyzed in positive MRM mode. Methodology was validated in accordance with the European Commission Decision 2002/657/EC. Performance characteristics permit the use of this methodology for steroid determination in animal serum samples. (C) 2009 Elsevier B.V. All rights reserved

引用

页码：2457 / 2464

页数：8

共 45 条

[21] Cryogenic grinding pre-treatment improves extraction efficiency of fluoroquinolones for HPLC-MS/MS determination in animal tissue [J].

Lolo, Manuel ;

Pedreira, Sandra ;

Vazquez, Beatriz I. ;

Franco, Carlos M. ;

Cepeda, Alberto ;

Fente, Cristina A. .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2007, 387 (05) :1933-1937

[22] Steroid profiling by tandem mass spectrometry improves the positive predictive value of newborn screening for congenital adrenal hyperplasia [J].

Minutti, CZ ;

Lacey, JM ;

Magera, MJ ;

Hahn, SH ;

McCann, M ;

Schulze, A ;

Cheillan, D ;

Dorche, C ;

Chace, DH ;

Lymp, JF ;

Zimmerman, D ;

Rinaldo, P ;

Matern, D .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2004, 89 (08) :3687-3693

[23] Determination of sulfates of androsterone and epiandrosterone liquid in human serum using isotope diluted chromatography-electrospray ionization-mass spectrometry [J].

Mitamura, K ;

Setaka, M ;

Shimada, K ;

Honma, S ;

Namiki, M ;

Koh, E ;

Mizokami, A .

BIOMEDICAL CHROMATOGRAPHY, 2005, 19 (10) :796-801

[24] Low serum testosterone assayed by liquid chromatography-tandem mass spectrometry.: Comparison with five immunoassay techniques [J].

Moal, Valerie ;

Mathieu, Elisabeth ;

Reynier, Pascal ;

Malthiery, Yves ;

Gallois, Yves .

CLINICA CHIMICA ACTA, 2007, 386 (1-2) :12-19

[25] Plasma biomarker profiling in the detection of growth promoter use in calves [J].

Mooney, M. H. ;

Situ, C. ;

Cacciatore, G. ;

Hutchinson, T. ;

Elliott, C. ;

Bergwerff, A. A. .

BIOMARKERS, 2008, 13 (03) :246-256

[26] Screening for estrogen residues in calf urine: Comparison of a validated yeast estrogen bioassay and gas chromatography-tandem mass spectrometry [J].

Nielen, M. W. F. ;

Bovee, T. F. H. ;

Heskamp, H. H. ;

Lasaroms, J. J. P. ;

Sanders, M. B. ;

Van Rhijn, J. A. ;

Groot, M. J. ;

Hoogenboom, L. A. P. .

FOOD ADDITIVES AND CONTAMINANTS, 2006, 23 (11) :1123-1131

[27] Novel analytical methods for the determination of steroid hormones in edible matrices [J].

Noppe, H. ;

Le Bizec, B. ;

Verheyden, K. ;

De Brabander, H. F. .

ANALYTICA CHIMICA ACTA, 2008, 611 (01) :1-16

[28] Liquid chromatography-electrospray ionization-mass Spectrometry method in multiple reaction MonitoringMode to determine 17α-ethynylestradiol residues in cattle hair without previous digestion [J].

Pedreira, S. ;

Lolo, M. ;

Vazquez, B. I. ;

Franco, C. M. ;

Cepeda, A. ;

Fente, C. .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2007, 55 (23) :9325-9329

[29] Occurrence of steroid estrogens, endocrine-disrupting phenols, and acid pharmaceutical residues in urban riverine water of the Pearl River Delta, South China [J].

Peng, Xianzhi ;

Yu, Yiyi ;

Tang, Caiming ;

Tan, Jianhua ;

Huang, Qiuxin ;

Wang, Zhendi .

SCIENCE OF THE TOTAL ENVIRONMENT, 2008, 397 (1-3) :158-166

[30]

*RIVM, 2007, CRL VIEW STAT ART AN

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