Quantitative LC-MS/MS method for the sensitive and simultaneous determination of natural hormones in bovine serum

被引：42
作者：
Regal, P. [1 ]
Vazquez, B. I. [1 ]
Franco, C. M. [1 ]
Cepeda, A. [1 ]
Fente, C. [1 ]
机构：
[1] Univ Santiago de Compostela, Fac Vet, Lugo 27002, Spain
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2009年 / 877卷 / 24期
关键词：
Hormone; LC-MS/MS; Bovine serum; TANDEM MASS-SPECTROMETRY; PERFORMANCE LIQUID-CHROMATOGRAPHY; ELECTROSPRAY-IONIZATION; STEROID-HORMONES; DERIVATIZATION; ESTROGENS; RESIDUES; TESTOSTERONE; PLASMA; ASSAY;
D O I：
10.1016/j.jchromb.2009.06.025
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We have developed a liquid chromatography tandem mass spectrometric (LC-MS/MS) method for the simultaneous quantitative analysis of several free forms of steroid hormones in bovine serum I pregnenolone (P-5), progesterone (P-4), 17hydroxyP(5), 17hydroxyP(4), testosterone (T), dehydroepiandrosterone (DHEA), androstenedione (A), estrone (E-1), 2,4 and 16 hydroxyE(1), 2 and 4 methoxyE(1)]. Deuterated analogs were used as internal standards. Serum proteins were eliminated with acetonitrile. Oxime derivatives of steroids were extracted with tert-butylmethylether and analyzed in positive MRM mode. Methodology was validated in accordance with the European Commission Decision 2002/657/EC. Performance characteristics permit the use of this methodology for steroid determination in animal serum samples. (C) 2009 Elsevier B.V. All rights reserved
引用
收藏
页码：2457 / 2464
页数：8
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