Long-term fertilization affects functional soil organic carbon protection mechanisms in a profile of Chinese loess plateau soil

Crop productivity and soil health are limited by organic carbon (OC), however, the variations in the mechanisms of SOC preservation in a complete soil profile subjected to long-term fertilization remains unclear. The objective of the study was to examined the content and profile distribution of the distinctive SOC protection mechanisms on a complete profile (0-100 cm) of Eumorthic Anthrosols in Northwest China after 23 years of chemical and manure fertilization. The soil was fractionated by combined physical-chemical and density floatation techniques. Throughout the profile, significant variations were observed among fractions. In the topsoil (0-20 and 20-40 cm), mineral coupling with the fertilization of manure (MNPK) enhanced total SOC content and recorded for 29% of SOC in the 0-20 and 20-40 cm layers. Moreover, MNPK increased the SOC content of the unprotected cPOC fraction by 60.9% and 61.5% in the 0-20 and 20-40 cm layer, while SOC content was low in the subsoil layers (40-60, 60-80 and 80 -100 cm, respectively) compared with the control (C). The highest OC under MNPK in physically protected micro-aggregates (mu agg) (6.36 and 6.06 g C kg(-1)), and occluded particulate organic carbon (iPOC) (1.41 and 1.29 g C kg(-1)) was found in the topsoil layers. The unprotected cPOC fraction was the greatest C accumulating fraction in the topsoil layers, followed by mu agg and H-mu Silt fractions in the soil profile, implying that these fractions were the most sensitive to the fertilization treatments. Overall, the unprotected, physically protected, and physico-chemically protected fractions were the dominant fractions for the sequestration of carbon across fertilization treatments and soil layers. (C) 2020 Elsevier Ltd. All rights reserved.