Evaluation of hematology, general serum biochemistry, bone turnover markers and bone marrow cytology in a glucocorticoid treated ovariectomized sheep model for osteoporosis research

被引:4
作者
Coelho, Catarina A. [1 ]
Bordelo, Joao P. [2 ]
Camassa, Jose A. [2 ]
Barros, Vera A. [3 ,4 ]
Babo, Pedro S. [3 ,4 ]
Gomes, Manuela E. [3 ,4 ]
Reis, Rui L. [3 ,4 ]
De Azevedo, Jorge T. [5 ,6 ]
Requicha, Joao F. [2 ,6 ]
Faisca, Pedro [1 ,7 ]
Carvalho, Pedro P. [8 ,9 ]
Viegas, Carlos A. [2 ,3 ,4 ,10 ]
Dias, Isabel R. [2 ,3 ,4 ,10 ]
机构
[1] Univ Lusofona Humanidades & Tecnol ULHT, Fac Vet Med FMV, Campo Grande 376, P-1749024 Lisbon, Portugal
[2] Univ Tras Os Montes & Alto Douro UTAD, Sch Agr & Vet Sci ECAV, Dept Vet Sci, P-5000801 Vila Real, Portugal
[3] Univ Minho, Res Grp 3Bs, Res Inst Biomat Biodegradables & Biomimet I3Bs, Headquarters European Inst Excellence Tissue Engn, AvePk,Parque Ciencia & Tecnol, P-4805017 Barco, Guimaraes, Portugal
[4] PT Govt Associate Lab ICVS 3Bs, P-4805017 Braga, Portugal
[5] UTAD, Dept Anim Sci, ECAV, P-5000801 Vila Real, Portugal
[6] UTAD, CECAV Ctr Anim Sci & Vet Studies, P-5000801 Vila Real, Portugal
[7] ULHT, Res Ctr Biosci & Hlth Technol, FMV, Campo Grande 376, P-1749024 Lisbon, Portugal
[8] Univ Sch Vasco da Gama EUVG, Dept Vet Med, Av Jose R Sousa Fernandes 197, P-3020210 Coimbra, Portugal
[9] EUVG, CIVG Vasco da Gama Res Ctr, P-3020210 Coimbra, Portugal
[10] UTAD, CITAB Ctr Res & Technol Agroenvironm & Biol Sci, P-5000801 Vila Real, Portugal
来源
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS | 2020年 / 92卷 / 04期
关键词
animal model; blood analysis; bone marrow cytology; lumbar vertebral microstructure; osteoporosis; sheep; LARGE ANIMAL-MODEL; MECHANICAL-PROPERTIES; RECEPTOR ACTIVATOR; FRACTURE; RESORPTION;
D O I
10.1590/0001-3765202020200435
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Osteoporosis is a metabolic disorder characterized by a loss of bone mass and structure and increasing the risk of fragility fractures, mostly among postmenopausal women. Sheep is a recognized large animal model for osteoporosis research. An experimental group of ewes (3-4 years old) was subjected to ovariectomy (OVX) and weekly glucocorticoid (GC) application for 24 weeks and compared with a sham control group. Blood and bone marrow parameters were analyzed before and 24 weeks after OVX and GC administration. Osteopenia was confirmed through micro-computed tomography and histomorphometric analysis of L4 vertebra in the study end. A statistically significant increase was observed in mean corpuscular volume, mean cell hemoglobin and monocytes and a decrease in red blood count and eosinophils (p<0.05). Alkaline phosphatase (ALP), gamma-glutamyl transpeptidase, magnesium and al-globulin increased, and creatinine, albumin, sodium and estradiol decreased (p<0.05). A slight decrease of bone formation markers (bone ALP and osteocalcin) and an increase of bone resorption markers (C-terminal telopeptides of collagen type 1 and tartrate-resistant acid phosphatase) were observed, but without statistical significance. This study aims to contribute to better knowledge of sheep as a model for osteoporosis research and the consequences that a performed induction protocol may impose on organic metabolism.
引用
收藏
页码:1 / 16
页数:16
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