Evaluation of hematology, general serum biochemistry, bone turnover markers and bone marrow cytology in a glucocorticoid treated ovariectomized sheep model for osteoporosis research

被引：4

作者：

Coelho, Catarina A. ^{[1
]}

Bordelo, Joao P. ^{[2
]}

Camassa, Jose A. ^{[2
]}

Barros, Vera A. ^{[3
,4
]}

Babo, Pedro S. ^{[3
,4
]}

Gomes, Manuela E. ^{[3
,4
]}

Reis, Rui L. ^{[3
,4
]}

De Azevedo, Jorge T. ^{[5
,6
]}

Requicha, Joao F. ^{[2
,6
]}

Faisca, Pedro ^{[1
,7
]}

Carvalho, Pedro P. ^{[8
,9
]}

Viegas, Carlos A. ^{[2
,3
,4
,10
]}

Dias, Isabel R. ^{[2
,3
,4
,10
]}

机构：

[1] Univ Lusofona Humanidades & Tecnol ULHT, Fac Vet Med FMV, Campo Grande 376, P-1749024 Lisbon, Portugal

[2] Univ Tras Os Montes & Alto Douro UTAD, Sch Agr & Vet Sci ECAV, Dept Vet Sci, P-5000801 Vila Real, Portugal

[3] Univ Minho, Res Grp 3Bs, Res Inst Biomat Biodegradables & Biomimet I3Bs, Headquarters European Inst Excellence Tissue Engn, AvePk,Parque Ciencia & Tecnol, P-4805017 Barco, Guimaraes, Portugal

[4] PT Govt Associate Lab ICVS 3Bs, P-4805017 Braga, Portugal

[5] UTAD, Dept Anim Sci, ECAV, P-5000801 Vila Real, Portugal

[6] UTAD, CECAV Ctr Anim Sci & Vet Studies, P-5000801 Vila Real, Portugal

[7] ULHT, Res Ctr Biosci & Hlth Technol, FMV, Campo Grande 376, P-1749024 Lisbon, Portugal

[8] Univ Sch Vasco da Gama EUVG, Dept Vet Med, Av Jose R Sousa Fernandes 197, P-3020210 Coimbra, Portugal

[9] EUVG, CIVG Vasco da Gama Res Ctr, P-3020210 Coimbra, Portugal

[10] UTAD, CITAB Ctr Res & Technol Agroenvironm & Biol Sci, P-5000801 Vila Real, Portugal

来源：

ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS | 2020年 / 92卷 / 04期

关键词：

animal model; blood analysis; bone marrow cytology; lumbar vertebral microstructure; osteoporosis; sheep; LARGE ANIMAL-MODEL; MECHANICAL-PROPERTIES; RECEPTOR ACTIVATOR; FRACTURE; RESORPTION;

D O I：

10.1590/0001-3765202020200435

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Osteoporosis is a metabolic disorder characterized by a loss of bone mass and structure and increasing the risk of fragility fractures, mostly among postmenopausal women. Sheep is a recognized large animal model for osteoporosis research. An experimental group of ewes (3-4 years old) was subjected to ovariectomy (OVX) and weekly glucocorticoid (GC) application for 24 weeks and compared with a sham control group. Blood and bone marrow parameters were analyzed before and 24 weeks after OVX and GC administration. Osteopenia was confirmed through micro-computed tomography and histomorphometric analysis of L4 vertebra in the study end. A statistically significant increase was observed in mean corpuscular volume, mean cell hemoglobin and monocytes and a decrease in red blood count and eosinophils (p<0.05). Alkaline phosphatase (ALP), gamma-glutamyl transpeptidase, magnesium and al-globulin increased, and creatinine, albumin, sodium and estradiol decreased (p<0.05). A slight decrease of bone formation markers (bone ALP and osteocalcin) and an increase of bone resorption markers (C-terminal telopeptides of collagen type 1 and tartrate-resistant acid phosphatase) were observed, but without statistical significance. This study aims to contribute to better knowledge of sheep as a model for osteoporosis research and the consequences that a performed induction protocol may impose on organic metabolism.

引用

页码：1 / 16

页数：16

共 47 条

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