Iterative In Situ Click Chemistry Creates Antibody-like Protein-Capture Agents

被引：95

作者：

Agnew, Heather D. ^{[1
]}

Rohde, Rosemary D. ^{[1
]}

Millward, Steven W. ^{[1
]}

Nag, Arundhati ^{[1
]}

Yeo, Woon-Seok ^{[1
]}

Hein, Jason E. ^{[2
,3
]}

Pitram, Suresh M. ^{[2
,3
]}

Tariq, Abdul Ahad ^{[1
]}

Burns, Vanessa M. ^{[1
]}

Krom, Russell J. ^{[1
]}

Fokin, Valery V. ^{[2
,3
]}

Sharpless, K. Barry ^{[2
,3
]}

Heath, James R. ^{[1
]}

机构：

[1] CALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA

[2] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

[3] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2009年 / 48卷 / 27期

基金：

加拿大自然科学与工程研究理事会;

关键词：

click chemistry; combinatorial chemistry; inhibitors; peptides; protein-capture agents; CARBONIC-ANHYDRASE; APTAMER; INHIBITORS; ACETYLCHOLINESTERASE; HYDROLYSIS; MOLECULES; LIBRARIES; SELECTION; VITRO; ACIDS;

D O I：

10.1002/anie.200900488

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Special agents for protein capture: Iterative in situ click chemistry (see scheme for the tertiary ligand screen) and the one-bead-one-compound method for the creation of a peptide library enable the fragment-based assembly of selective high-affinity protein-capture agents. The resulting ligands are water-soluble and stable chemically, biochemically, and thermally. They can be produced in gram quantities through copper(I)-catalyzed cycloaddition. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：4944 / 4948

页数：5

共 35 条

[1] Isolation of protein ligands from large peptoid libraries
Alluri, PG
Reddy, MM
Bachhawat-Sikder, K
Olivos, HJ
Kodadek, T
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (46) : 13995 - 14004
[2] Antibodies: The generation game
Blow, Nathan
[J]. NATURE, 2007, 447 (7145) : 741 - +
[3] Antibodies in diagnostics - from immunoassays to protein chips
Borrebaeck, CAK
[J]. IMMUNOLOGY TODAY, 2000, 21 (08): : 379 - 382
[4] Freeze-frame inhibitor captures acetylcholinesterase in a unique conformation
Bourne, Y
Kolb, HC
Radic, Z
Sharpless, KB
Taylor, P
Marchot, P
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (06) : 1449 - 1454
[5] Direct in vitro selection of a 2′-O-methyl aptamer to VEGF
Burmeister, PE
Lewis, SD
Silva, RF
Preiss, JR
Horwitz, LR
Pendergrast, PS
McCauley, TG
Kurz, JC
Epstein, DM
Wilson, C
Keefe, AD
[J]. CHEMISTRY & BIOLOGY, 2005, 12 (01): : 25 - 33
[6] KINETIC RESOLUTION OF UNNATURAL AND RARELY OCCURRING AMINO-ACIDS - ENANTIOSELECTIVE HYDROLYSIS OF N-ACYL AMINO-ACIDS CATALYZED BY ACYLASE-I
CHENAULT, HK
DAHMER, J
WHITESIDES, GM
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1989, 111 (16) : 6354 - 6364
[7] Automated selection of aptamers against protein targets translated in vitro:: from gene to aptamer -: art. no. e108
Cox, JC
Hayhurst, A
Hesselberth, J
Bayer, TS
Georgiou, G
Ellington, AD
[J]. NUCLEIC ACIDS RESEARCH, 2002, 30 (20) : e108
[8] GENERAL-METHOD FOR RAPID SYNTHESIS OF MULTICOMPONENT PEPTIDE MIXTURES
FURKA, A
SEBESTYEN, F
ASGEDOM, M
DIBO, G
[J]. INTERNATIONAL JOURNAL OF PEPTIDE AND PROTEIN RESEARCH, 1991, 37 (06): : 487 - 493
[9] SCREENING CHEMICALLY SYNTHESIZED PEPTIDE LIBRARIES FOR BIOLOGICALLY-RELEVANT MOLECULES
GEYSEN, HM
MASON, TJ
[J]. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 1993, 3 (03) : 397 - 404
[10] DIVERSITY OF OLIGONUCLEOTIDE FUNCTIONS
GOLD, L
POLISKY, B
UHLENBECK, O
YARUS, M
[J]. ANNUAL REVIEW OF BIOCHEMISTRY, 1995, 64 : 763 - 797

← 1 2 3 4 →