Verticine, ebeiedine and suchengbeisine isolated from the bulbs of Fritillaria thunbergii Miq. inhibited the gene expression and production of MUC5AC mucin from human airway epithelial cells

Background: The bulb of Fritillorin thunhergii has been utilised as mucoregulators and expectorants for controlling the airway inflammatory diseases in folk medicine. Hypothesis/Purpose: We investigated whether verticine, ebeiedine and suchengbeisine isolated from the bulbs of Fritillaria thunbergii inhibit the gene expression and production of MUC5AC mucin from human airway epithelial cells. Study design: Confluent NCI-H292 cells were pretreated with verticine, ebeiedine or suchengbeisine for 30 min and then stimulated with EGF, PMA or TNF-alpha for 24h. The MUC5AC mucin gene expression was measured by RT-PCR. Production of MUC5AC mucin protein was measured by ELISA. Results: (1) Verticine, ebeiedine or suchengbeisine inhibited the expression of MUC5AC mucin gene induced by EGF, PMA or TNF-alpha: (2) The production of MUC5AC mucin protein induced by EGF, PMA or TNF-alpha were also inhibited by treatment of verticine, ebeiedine or suchengbeisine. Conclusion: These results suggest that verticine, ebeiedine and suchengbeisine isolated from the bulbs of Fritillorio thunbergii inhibit the gene expression and production of MUC5AC mucin, by directly acting on airway epithelial cells, and the results are consistent with the traditional use of Fritillurin thunbergii as remedy for diverse inflammatory pulmonary diseases. CO 2016 Elsevier GmbH. All rights reserved.