Structural characterization of phenolic compounds and antioxidant activity of the phenolic-rich fraction from defatted adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) seed meal

被引：63

作者：

Wang, Lifeng ^{[1
]}

Chen, Chao ^{[1
]}

Su, Anxiang ^{[1
]}

Zhang, Yiyi ^{[1
]}

Yuan, Jian ^{[1
]}

Ju, Xingrong ^{[1
]}

机构：

[1] Nanjing Univ Finance & Econ, Key Lab Grains & Oils Qual Control & Proc, Collaborat Innovat Ctr Modern Grain Circulat & Sa, Coll Food Sci & Engn, Nanjing 210023, Jiangsu, Peoples R China

来源：

FOOD CHEMISTRY | 2016年 / 196卷

基金：

中国国家自然科学基金;

关键词：

Defatted adlay seed meal; Fractionation; Phenolic compounds; Antioxidant activity; Ferulic acid; Rutin; Quercetin; IN-VITRO; FLAVONOIDS; PROFILES; ASSAY; SUPPRESSION; EXTRACTS; CELLS; LUNG; VIVO;

D O I：

10.1016/j.foodchem.2015.09.083

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The current study aims to investigate the antioxidant activities of various extracts from defatted adlay seed meal (DASM) based on the oxygen radical absorbance capacity (ORAC) assay, peroxyl radical scavenging capacity (PSC) assay and cellular antioxidant activity (CAA) assay. Of all the fractions, the n-butanol fraction exhibited the highest antioxidant activity, followed by crude acetone extract and aqueous fractions. Of the three sub-fractions obtained by Sephadex LH-20 chromatography, subfraction 3 possessed the highest antioxidant activity and total phenolic content. There was a strong positive correlation between the total phenolic content and the antioxidant activity. Based on HPLC-DAD-ESI-MS/MS analysis, the most abundant phenolic acid in sub-fraction 3 of DASM was ferulic acid at 67.28 mg/g, whereas the predominant flavonoid was rutin at 41.11 mg/g. Of the major individual compounds in sub-fraction 3, p-coumaric acid exhibited the highest ORAC values, and quercetin exhibited the highest PSC values and CAA values. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：509 / 517

页数：9

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