Design of a Diagnostic Immunoassay for Aflatoxin M1 Based on a Plant-Produced Antibody

被引：4

作者：

Capodicasa, Cristina ^{[1
]}

Bastiani, Erica ^{[2
]}

Serra, Thea ^{[3
]}

Anfossi, Laura ^{[3
]}

Catellani, Marcello ^{[4
]}

机构：

[1] Casaccia Res Ctr, Dept Sustainabil, Lab Biotechnol, ENEA, Via Anguillarese 301, I-00123 Rome, Italy

[2] Euroclone SpA, Via Figino 20 22, I-20016 Milan, Italy

[3] Univ Turin, Dept Chem, Via Giuria 7, I-10125 Turin, Italy

[4] Casaccia Res Ctr, Dept Sustainabil, Lab Bio Prod & Bio Proc, ENEA, Via Anguillarese 301, I-00123 Rome, Italy

来源：

TOXINS | 2022年 / 14卷 / 12期

关键词：

aflatoxin M1; plant molecular farming; ELISA; recombinant antibody; Nicotiana benthamiana; transient expression; food safety; TRANSIENT EXPRESSION; MONOCLONAL-ANTIBODY; M-1; VALIDATION; MYCOTOXINS;

D O I：

10.3390/toxins14120851

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A new green competitive ELISA for aflatoxin M1 quantification in raw milk was developed. This diagnostic tool is based on an anti AFM1 mAb produced by plant molecular farming in alternative to classical systems. Our assay, showing an IC50 below 25 ng/L, fits with the requirements of EU legislation limits for AFM1 (50 ng/L). Optimal accuracy was achieved in correspondence of the decision levels (25 and 50 ng/L), and the assay enabled AFM1 quantification in the range 5-110 ng/L, with limit of detection 3 ng/L. Moreover, to evaluate a real applicability in diagnostics, raw milk-spiked samples were analysed, achieving satisfactory recovery rates of AFM1. In conclusion, an efficient and ready-to-use diagnostic assay for the quantification of aflatoxin M1 in milk, based on a plant-produced recombinant mAb, has been successfully developed.

引用

页数：17

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