The aim of the present in vitro study was the evaluation of new formulation toothpastes on preventing dentin erosion produced by a soft drink (Coca Cola (R)), using atomic force microscopy (AFM) and scanning electron microscopy (SEM). Fifty dentin specimens were divided in treatment and control halves and were than assigned to 5 groups of 10 specimens each: group 1a: intact dentin, group 1b: dentin + soft drink, group 2a: intact dentin + Biorepair Plus-Sensitive Teeth (R), group 2b: dentin + soft drink + Biorepair Plus-Sensitive Teeth (R), group 3a: intact dentin + Biorepair Plus-Total Protection (R), group 3b: dentin + soft drink + Biorepair Plus-Total Protection (R), group 4a: intact dentin + Sensodyne Repair & Protect (R), group 4b: dentin + soft drink + Sensodyne Repair & Protect (R), group 5a: intact dentin + Colgate Sensitive Pro Relief (R), group 5b: dentin + soft drink + Colgate Sensitive Pro Relief (R). The surface of each specimen was imaged by AFM and SEM. Comparing specimens of group a and b (no demineralization and demineralization), a statistically significant difference (p < 0.01) in R-rms values was registered. Comparing b groups, all the analyzed toothpastes tended to remineralize the dentine surface in different extent. Biorepair Plus-Total Protection (R) and Sensodyne Repair & Protect (R) provided higher protective effect against dentin demineralization. (C) 2013 Wiley Periodicals, Inc.