Protein Profiling of Extracellular Vesicles Associated With Cisplatin Resistance in Lung Cancer

被引：9
作者：
Balbinotti, Helier [1 ]
Cadore, Nathan A. [1 ]
Dutra, Cristine S. [1 ]
da Silva, Edileuza D. [1 ]
Ferreira, Henrique B. [1 ,2 ]
Zaha, Arnaldo [1 ,2 ]
Monteiro, Karina M. [1 ,2 ]
机构：
[1] Univ Fed Rio Grande do Sul, Ctr Biotecnol, Lab Genom Estrutural & Func, Porto Alegre, RS, Brazil
[2] Univ Fed Rio Grande do Sul, Inst Biociencias, Dept Biol Mol & Biotecnol, Porto Alegre, RS, Brazil
来源：
ANTICANCER RESEARCH | 2020年 / 40卷 / 10期
关键词：
Extracellular vesicles; lung cancer; drug resistance; cisplatin; mass spectrometry; EXOSOMES; CHEMOTHERAPY; GROWTH;
D O I：
10.21873/anticanres.14563
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Background/Aim: Extracellular vesicles (EVs) can mediate drug resistance within the tumor microenvironment by delivering bioactive molecules, including proteins. Here, we performed a comparative proteomic analysis of EVs secreted by A549 lung cancer cells and their cisplatin-resistant counterparts in order to identify proteins involved in drug resistance. Materials and Methods: Cells were co-cultivated using a transwell system to evaluate EV exchange. EVs were isolated by ultracentrifugation and analyzed using microscopy and nanoparticle tracking. EV proteome was analyzed by mass spectrometry. Results: EV-mediated communication was observed between co-cultured A549 and A549/CDDP cells. EVs isolated from both cells were mainly exosome-like structures. Extracellular matrix components, cell adhesion proteins, complement factors, histones, proteasome subunits and membrane transporters were found enriched in the EVs released by cisplatin-resistant cells. Conclusion: Proteins identified in this work may have a relevant role in modulating the chemosensitivity of the recipient cells and could represent useful biomarkers to monitor cisplatin response in lung cancer.
引用
收藏
页码：5509 / 5516
页数：8
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