Immunolocalization of steroidogenic enzymes (P450scc, P450c17, P450arom) in gonad of Japanese eel

被引:6
作者
Ijiri, S [1 ]
Takei, N [1 ]
Andachi, S [1 ]
Yamauchi, K [1 ]
机构
[1] Hokkaido Univ, Grad Sch Fisheries Sci, Hakodate, Hokkaido, Japan
关键词
Recombinant Protein; Outer Layer; Leydig Cell; Ovarian Follicle; Immunoreactive Cell;
D O I
10.1023/B:FISH.0000030530.28922.d7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Antibodies against P450scc, P450c17 and P450arom were generated using recombinant proteins. In eel testis, P450scc and P450c17 were immunolocalized as clusters in Leydig cells. In vitellogenic eel ovary, P450scc and P450c17 immunoreactive cells were localized as clusters in the outer layer of the ovarian follicle. In contrast, P450arom seemed to be immunolocalized in the innermost follicle layer.
引用
收藏
页码:209 / 210
页数:2
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