Stem-loop oligonucleotides: a robust tool for molecular biology and biotechnology

被引：125
作者：
Broude, NE [1 ]
机构：
[1] Boston Univ, Ctr Adv Biotechnol, Boston, MA 02215 USA
[2] Boston Univ, Dept Biomed Engn, Boston, MA 02215 USA
来源：
TRENDS IN BIOTECHNOLOGY | 2002年 / 20卷 / 06期
关键词：
D O I：
10.1016/S0167-7799(02)01942-X
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The specific structural features of stem-loop (hairpin) DNA constructs provide increased specificity of target recognition. Recently, several robust assays have been developed that exploit the potential of structurally constrained oligonucleotides to hybridize with their cognate targets. Here, I review new diagnostic approaches based on the formation of stem-loop DNA oligonucleotides: molecular beacon methodology, suppression PCR approaches and the use of hairpin probes in DNA microarrays. The advantages of these techniques over existing ones for sequence-specific DNA detection, amplification and manipulation are discussed.
引用
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页码：249 / 256
页数：8
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