Mass spectrometry of extracellular vesicles

被引：106

作者：

Pocsfalvi, Gabriella ^{[1
]}

Stanly, Christopher ^{[1
]}

Vilasi, Annalisa ^{[1
]}

Fiume, Immacolata ^{[1
]}

Capasso, Giovambattista ^{[2
]}

Turiak, Lilla ^{[3
]}

Buzas, Edit I. ^{[4
]}

Vekey, Karoly ^{[3
]}

机构：

[1] Natl Res Council Italy, Inst Biosci & BioResources, Mass Spectrometry & Prote, Via P Castellino 111, I-80131 Naples, Italy

[2] Univ Naples 2, Div Nephrol, Dept Cardiovasc Sci, Naples, Italy

[3] Hungarian Acad Sci, Inst Organ Chem, Mass Spectrometry Prote Grp, Res Ctr Nat Sci, Budapest, Hungary

[4] Semmelweis Univ, Dept Genet Cell & Immunobiol, H-1085 Budapest, Hungary

来源：

MASS SPECTROMETRY REVIEWS | 2016年 / 35卷 / 01期

关键词：

extracellular vesicles; subcellular proteomics; proteins; lipids; biomarker discovery; CELL-DERIVED EXOSOMES; QUANTITATIVE PROTEOMIC ANALYSIS; URINARY EXOSOMES; MEMBRANE-VESICLES; INTERCELLULAR TRANSFER; DIABETIC-NEPHROPATHY; APOPTOTIC BODIES; EMERGING ROLE; MICROVESICLES; PROTEIN;

D O I：

10.1002/mas.21457

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

The review briefly summaries main features of extracellular vesicles, a joint terminology for exosomes, microvesicles, and apoptotic vesicles. These vesicles are in the center of interest in biology and medical sciences, and form a very active field of research. Mass spectrometry (MS), with its specificity and sensitivity, has the potential to identify and characterize molecular composition of these vesicles; but as yet there are only a limited, but fast-growing, number of publications that use MS workflows in this field. MS is the major tool to assess protein composition of extracellular vesicles: qualitative and quantitative proteomics approaches are both reviewed. Beside proteins, lipid and metabolite composition of vesicles might also be best assessed by MS techniques; however there are few applications as yet in this respect. The role of alternative analytical approaches, like gel-based proteomics and antibody-based immunoassays, are also mentioned. The objective of the review is to give an overview of this fast-growing field to help orient MS-based research on extracellular vesicles. (c) 2015 Wiley Periodicals, Inc.

引用

页码：3 / 21

页数：19

共 166 条

[61] Microvesicle and tunneling nanotube mediated intercellular transfer of g-protein coupled receptors in cell cultures [J].