Uncharged Gemini-Amphiphiles as Components of Cationic Liposomes for Delivery of Nucleic Acids

被引：2

作者：

Shmendel, E., V ^{[1
]}

Bakhareva, S. A. ^{[1
]}

Makarova, D. M. ^{[1
]}

Chernikov, I., V ^{[2
]}

Morozova, N. G. ^{[1
]}

Chernolovskaya, E. L. ^{[2
]}

Zenkova, M. A. ^{[2
]}

Maslov, M. A. ^{[1
]}

机构：

[1] MIREA Russian Technol Univ, Lomonosov Inst Fine Chem Technol, Moscow 119571, Russia

[2] Russian Acad Sci, Inst Chem Biol & Fundamental Med, Siberian Branch, Novosibirsk 630090, Russia

来源：

RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY | 2020年 / 46卷 / 06期

关键词：

uncharged gemini-amphiphiles; gene therapy; cationic liposomes; nucleic acids; cytotoxicity; transfection; POLYMERIC MICELLES; TARGETED DELIVERY; GENE DELIVERY; DESIGN; LIPIDS; TOXICITY; LIGAND; CANCER;

D O I：

10.1134/S106816202006031X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

New uncharged gemini-amphiphiles have been synthesized. A series of cationic liposomes based on the polycationic amphiphile 1,26-bis(cholest-5-en-3 beta-yloxycarbonylamino)-7,11,16,20-tetraazahexacosan tetrahydrochloride (2X3), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) and uncharged gemini-amphiphiles that contain short hydrophobic and long hydrophilic spacers have been prepared. The physicochemical characteristics, cytotoxicity, and transfection activity of cationic liposomes have been studied. Cationic liposomes that include 10% (mol) of a uncharged gemini-amphiphile with the oligooxymethylene spacer have formed small complexes with plasmid DNA and efficiently delivered it into eukaryotic cells.

引用

页码：1250 / 1260

页数：11

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