Preparation and characterization of antiadhesion barrier film from hyaluronic acid-grafted electrospun poly(caprolactone) nanofibrous membranes for prevention of flexor tendon postoperative peritendinous adhesion

被引：72

作者：

Chen, Shih-Hsien ^{[1
]}

Chen, Chih-Hao ^{[1
,2
]}

Shalumon, K. T. ^{[1
]}

Chen, Jyh-Ping ^{[1
,3
]}

机构：

[1] Chang Gung Univ, Coll Med, Chang Gung Mem Hosp, Dept Chem & Mat Engn, Taoyuan 333, Taiwan

[2] Chang Gung Univ, Coll Med, Chang Gung Mem Hosp, Dept Plast & Reconstruct Surg, Taoyuan 333, Taiwan

[3] Chang Gung Univ Sci & Technol, Res Ctr Ind Human Ecol, Taoyuan, Taiwan

来源：

INTERNATIONAL JOURNAL OF NANOMEDICINE | 2014年 / 9卷

关键词：

peritendinous adhesion; hyaluronic acid; polycaprolactone; antiadhesion; nanofibrous membranes; barrier film; surface grafting; IN-VITRO; COMPOSITE NANOFIBERS; PERITONEAL ADHESIONS; SURFACE MODIFICATION; ABDOMINAL ADHESIONS; REPAIR; POLYCAPROLACTONE; IMMOBILIZATION; PROLIFERATION; VIVO;

D O I：

10.2147/IJN.S67931

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Peritendinous adhesion is one of the common complications encountered after tendon injury and subsequent surgery, and it can be minimized by introducing a physical barrier between the injured site and the surrounding tissue. An electrospun hyaluronic acid-grafted poly(caprolactone) (PCL-g-HA) nanofibrous membrane (NFM) is proposed as an alternative to current antiadhesion barrier films. HA is covalently grafted to surface-aminolyzed PCL nanofibers, using carbodiimide as the coupling agent. Pristine PCL and PCL-g-HA NFMs were characterized by scanning electron microscopy, thermogravimetric analysis, X-ray photoelectron spectroscopy, Fourier-transform infrared spectroscopy, and mechanical testing. In vitro cell culture with fibroblasts showed that PCL-g-HA NFMs reduced cellular adhesion on the membrane surface while maintaining cell proliferation. Animal experiments using a rabbit flexor digitorum profundus tendon model confirmed the efficacy of PCL-g-HA in reducing peritendinous adhesion, based on gross observation, histology, joint flexion-angle measurements, gliding tests, and biomechanical evaluation.

引用

页码：4079 / 4092

页数：14

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