Determination of gatifloxacin and ornidazole in tablet dosage forms by high-performance thin-layer chromatography

被引：41
|
作者：
Suhagia, Bhanubhai N. [1 ]
Shah, Shailesh A. [1 ]
Rathod, Ishwarsinh S. [1 ]
Patel, Haresh M. [1 ]
Shah, Dinesh R. [1 ]
Marolia, Bhavin P. [1 ]
机构：
[1] LM Coll Pharm, Dept Qual Assurance, Ahmadabad 380009, Gujarat, India
来源：
ANALYTICAL SCIENCES | 2006年 / 22卷 / 05期
关键词：
Silica gel;
D O I：
10.2116/analsci.22.743
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A simple and sensitive high-performance thin-layer chromatography (HPTLC) method has been developed for the quantitative estimation of gatifloxacin and ornidazole in its combined dosage forms. Gatifloxacin and ornidazole were chromatographed on silica Gel 60 F-254 TLC plate using n-butanol:methanol:ammonia (6 M) (8:1:1.5 v/v) as the mobile phase and scanned at 302 nm using a Camag TLC Scanner 3. The R-f value of gatifloxacin and ornidazole was found to be 0.21 +/- 0.02 and 0.76 +/- 0.04, respectively. The linearity of gatifloxacin and ornidazole were in the range of 100 - 500 ng/spot and 250 - 1250 ng/spot, respectively. The limit of detection was found to be 40 ng/spot for gatifloxacin and 100 ng/spot for ornidazole. The proposed method was applied for the determination of gatifloxacin and ornidazole in combined dosage forms.
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页码：743 / 745
页数：3
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