Neural progenitor cells from human induced pluripotent stem cells generated less autogenous immune response

被引：15

作者：

Huang Ke ^{[1
]}

Liu PengFei ^{[1
,2
]}

Li Xiang ^{[1
]}

Chen ShuBin ^{[1
]}

Wang LiHui ^{[1
]}

Qin Li ^{[3
]}

Su ZhengHui ^{[1
]}

Huang WenHao ^{[1
]}

Liu JuLi ^{[1
]}

Jia Bei ^{[4
]}

Liu Jie ^{[4
]}

Cai JingLei ^{[1
]}

Pei DuanQing ^{[1
]}

Pan GuangJin ^{[1
]}

机构：

[1] Chinese Acad Sci, Guangdong Prov Key Lab Stem Cell & Regenerat Med, Key Lab Regenerat Biol, Guangzhou Inst Biomed & Hlth, Guangzhou 510530, Guangdong, Peoples R China

[2] Jilin Univ, Sch Pharmaceut Sci, Dept Regenerat Med, Changchun 130021, Peoples R China

[3] Chinese Acad Sci, State Key Lab Resp Dis, Guangzhou Inst Biomed & Hlth, Lab Pathogen Biol,Ctr Infect & Immun, Guangzhou 510530, Guangdong, Peoples R China

[4] Southern Med Univ, Nanfang Hosp, Dept Obstet & Gynecol, Guangzhou 510515, Guangdong, Peoples R China

来源：

SCIENCE CHINA-LIFE SCIENCES | 2014年 / 57卷 / 02期

基金：

中国国家自然科学基金;

关键词：

induced pluripotent stem cells; immunogenicity; iPSC-derived neural progenitor cells; GENETIC CORRECTION; IPS CELLS; IMMUNOGENICITY; DIFFERENTIATION; FIBROBLASTS; MUTATION; MODEL;

D O I：

10.1007/s11427-013-4598-6

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The breakthrough development of induced pluripotent stem cells (iPSCs) raises the prospect of patient-specific treatment for many diseases through the replacement of affected cells. However, whether iPSC-derived functional cell lineages generate a deleterious immune response upon auto-transplantation remains unclear. In this study, we differentiated five human iPSC lines from skin fibroblasts and urine cells into neural progenitor cells (NPCs) and analyzed their immunogenicity. Through co-culture with autogenous peripheral blood mononuclear cells (PBMCs), we showed that both somatic cells and iPSC-derived NPCs do not stimulate significant autogenous PBMC proliferation. However, a significant immune reaction was detected when these cells were co-cultured with allogenous PBMCs. Furthermore, no significant expression of perforin or granzyme B was detected following stimulation of autogenous immune effector cells (CD3(+)CD8(-) T cells, CD3(+)CD8(+) T cells or CD3(-)CD56(+) NK cells) by NPCs in both PBMC and T cell co-culture systems. These results suggest that human iPSC-derived NPCs may not initiate an immune response in autogenous transplants, and thus set a base for further preclinical evaluation of human iPSCs.

引用

页码：162 / 170

页数：9

共 38 条

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