Structure of the α-polysaccharide of Proteus serogroup O34 containing 2-acetamido-2-deoxy-α-D-galactosyl phosphate

被引：7
作者：
Perepelov, AV
Kolodziejska, K
Kondakova, AN
Wykrota, M
Knirel, YA
Sidorczyk, Z
Rozalski, A
机构：
[1] Russian Acad Sci, ND Zelinskii Organ Chem Inst, Moscow 119991, Russia
[2] Univ Lodz, Inst Microbiol & Immunol, Dept Gen Microbiol, PL-90237 Lodz, Poland
[3] Univ Lodz, Inst Microbiol & Immunol, Dept Immunobiol Bacteria, PL-90237 Lodz, Poland
来源：
CARBOHYDRATE RESEARCH | 2004年 / 339卷 / 12期
基金：
俄罗斯基础研究基金会;
关键词：
Proteus vulgaris; Proteus mirabilis; lipopolysaccharide; O-antigen; bacterial polysaccharide structure; 2-acetamido-2-deoxygalactosyl phosphate;
D O I：
10.1016/j.carres.2004.06.005
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
On mild acid degradation of the lipopolysaccharide of Proteus vulgaris O34, strain CCUG 4669, the O-polysaccharide was cleaved at a glycosyl-phosphate linkage that is present in the main chain. The resultant phosphorylated oligosaccharides and an alkali-treated lipopolysaccharide were studied by sugar and methylation analyses along with H-1 and C-13 NMR spectroscopy, and the following structure of the branched tetrasaccharide phosphate repeating unit of the O-polysaccharide was established: [GRAPHICS] The O-polysaccharide of Proteus mirabilis strain TG 276 was found to have the same structure and, based on the structural and serological data, this strain was proposed to be classified into the same Proteus serogroup O34. (C) 2004 Elsevier Ltd. All rights reserved.
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收藏
页码：2145 / 2149
页数：5
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