CRISPR technologies for precise epigenome editing

被引：227
作者：
Nakamura, Muneaki [1 ]
Gao, Yuchen [1 ,2 ,5 ]
Dominguez, Antonia A. [1 ,6 ]
Qi, Lei S. [1 ,3 ,4 ]
机构：
[1] Stanford Univ, Dept Bioengn, Stanford, CA 94305 USA
[2] Stanford Univ, Canc Biol Program, Stanford, CA 94305 USA
[3] Stanford Univ, Dept Chem & Syst Biol, Stanford, CA 94305 USA
[4] Stanford Univ, Stanford ChEM H Inst, Stanford, CA 94305 USA
[5] Mammoth Biosci, San Francisco, CA USA
[6] Sana Biotechnol, San Francisco, CA USA
来源：
NATURE CELL BIOLOGY | 2021年 / 23卷 / 01期
基金：
美国国家科学基金会; 美国国家卫生研究院;
关键词：
DNA METHYLATION; REGULATORY ELEMENTS; GENE-EXPRESSION; CHROMATIN; ENHANCERS; IDENTIFICATION; ACTIVATION; THOUSANDS; BINDING; TARGET;
D O I：
10.1038/s41556-020-00620-7
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Qi and colleagues review CRISPR-based epigenome engineering technologies to modulate histone and DNA modifications and to perturb DNA and RNA regulatory elements and chromatin organization. The epigenome involves a complex set of cellular processes governing genomic activity. Dissecting this complexity necessitates the development of tools capable of specifically manipulating these processes. The repurposing of prokaryotic CRISPR systems has allowed for the development of diverse technologies for epigenome engineering. Here, we review the state of currently achievable epigenetic manipulations along with corresponding applications. With future optimization, CRISPR-based epigenomic editing stands as a set of powerful tools for understanding and controlling biological function.
引用
收藏
页码：11 / 22
页数：12
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