Genetic aspects of carbon catabolite repression of the STA2 glucoamylase gene in Saccharomyces cerevisiae

被引:0
|
作者
Kartasheva, NN [1 ]
Kuchin, SV [1 ]
Benevolensky, SV [1 ]
机构
[1] MOSCOW GENET & SELECT IND MICROORGANISMS INST,MOSCOW 113545,RUSSIA
关键词
yeast; extracellular glucoamylase; carbon catabolite repression;
D O I
10.1002/(SICI)1097-0061(199610)12:13<1297::AID-YEA13>3.0.CO;2-U
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Three regulatory genes, known to be required for glucose repression/derepression of some genes in Saccharomyces cerevisiae, were disrupted to study their effects on the carbon-source regulation of the STA2 glucoamylase gene expression. Using a STA2-lacZ fusion it was found that: (1) the MIGI gene is dispensable for the repression of the STA2 gene; (2) there are two components in the carbon-source repression of STA2: HXK2-dependent and HXK2-independent; and (3) the HAP2 gene seems to be involved in repression rather than activation of the STA2 expression.
引用
收藏
页码:1297 / 1300
页数:4
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