POLYMERASE CHAIN REACTION DETECTION OF RABBIT DNA IN FOOD AND ANIMAL FEED

被引：0
作者：
Martin, I [1 ]
Garcia, T. [1 ]
Fajardo, V [1 ]
Rojas, M. [1 ]
Pegels, N. [1 ]
Hernandez, P. E. [1 ]
Gonzalez, I [1 ]
Martin, R. [1 ]
机构：
[1] Univ Complutense Madrid, Fac Vet, Dept Nutr Bromatol & Tecnol Alimentos, E-28040 Madrid, Spain
来源：
WORLD RABBIT SCIENCE | 2009年 / 17卷 / 01期
关键词：
rabbit; feedstuff; PCR; species identification; 12S rRNA gene; SPECIES-SPECIFIC PCR; REAL-TIME PCR; MEAT-PRODUCTS; BONE MEAL; IDENTIFICATION; ORIGIN; ASSAY; RAW; AUTHENTICATION; FEEDSTUFFS;
D O I：
暂无
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
A polymerase chain reaction (PCR) based on oligonucleotide primers targeting the mitochondrial 12S rRNA gene was developed for the specific identification of rabbit DNA (Oryctolagus cuniculus) in food and feedstuffs. The specificity of the primers was verified by PCR analysis of DNA from 32 non-target species including mammals, birds, fish, and plant species. Analysis of experimental mixtures demonstrated the presence of rabbit-derived materials in the range of 0.1-100%. Prolonged heat treatment (up to 133 C for 20 min at 300 kPa) applied to rabbit muscle/oats binary mixtures did not affect the performance of the method, which could therefore be said to be very useful for the accurate identification of rabbit materials in products submitted to denaturing technologies when other methods are not suitable.
引用
收藏
页码：27 / 34
页数：8
相关论文
共 24 条
  • [1] Identification of meats using random amplified polymorphic DNA (RAPD) technique
    Arslan, A
    Ilhak, I
    Calicioglu, M
    Karahan, M
    [J]. JOURNAL OF MUSCLE FOODS, 2005, 16 (01) : 37 - 45
  • [2] The detection of horse and donkey using real-time PCR
    Chisholm, J
    Conyers, C
    Booth, C
    Lawley, W
    Hird, H
    [J]. MEAT SCIENCE, 2005, 70 (04) : 727 - 732
  • [3] Development of a DNA-based assay for species identification in meat and bone meal
    Colgan, S
    O'Brien, L
    Maher, M
    Shilton, N
    McDonnell, K
    Ward, S
    [J]. FOOD RESEARCH INTERNATIONAL, 2001, 34 (05) : 409 - 414
  • [4] A multiplex PCR assay for the identification of animal species in feedstuffs
    Dalmasso, A
    Fontanella, E
    Piatti, P
    Civera, T
    Rosati, S
    Bottero, MT
    [J]. MOLECULAR AND CELLULAR PROBES, 2004, 18 (02) : 81 - 87
  • [5] Duplex polymerase chain reaction for detection of pork meat in horse meat fresh sausages from Italian retail sources
    Di Pinto, A
    Forte, VT
    Conversano, MC
    Tantillo, GM
    [J]. FOOD CONTROL, 2005, 16 (05) : 391 - 394
  • [6] *EC COUNC, 2002, OFF J EUR COMM L, V273, P1
  • [7] *EC COUNC, 2001, OFF J EUR COMM L, V147
  • [8] PCR-RFLP authentication of meats from red deer (Cervus elaphus), fallow deer (Dama dama), roe deer (Capreolus capreolus), cattle (Bos taurus), sheep (Ovis aries), and goat (Capra hircus)
    Fajardo, V
    González, I
    López-Calleja, I
    Martín, I
    Hernández, PE
    García, T
    Martín, R
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2006, 54 (04) : 1144 - 1150
  • [9] Fajardo V, 2007, J AOAC INT, V90, P179
  • [10] Determination of the animal origin of raw food by species-specific PCR
    Herman, L
    [J]. JOURNAL OF DAIRY RESEARCH, 2001, 68 (03) : 429 - 436
123