Rapid exchange of A:T base pairs is essential for recognition of DNA homology by human Rad51 recombination protein

被引:67
作者
Gupta, RC
Folta-Stogniew, E
O'Malley, S
Takahashi, M
Radding, CM [1 ]
机构
[1] Yale Univ, Dept Genet, New Haven, CT 06510 USA
[2] Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT 06510 USA
[3] Univ Paris Sud, Inst Curie, CNRS,Unite Mixte Rech 216, Grp Etude Mutagenese & Cancerogenese, F-91405 Orsay, France
关键词
D O I
10.1016/S1097-2765(00)80381-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Human Rad51 belongs to a ubiquitous family of proteins that enable a single strand to recognize homology in duplex DNA, and thereby to initiate genetic exchanges and DNA repair, but the mechanism of recognition remains unknown. Kinetic analysis by fluorescence resonance energy transfer combined with the study of base substitutions and base mismatches reveals that recognition of homology, helix destabilization, exchange of base pairs, and initiation of strand exchange are integral parts of a rapid, concerted mechanism in which A:T base pairs play a critical role. Exchange of base pairs is essential for recognition of homology, and physical evidence indicates that such an exchange occurs early enough to mediate recognition.
引用
收藏
页码:705 / 714
页数:10
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