Zinc at pharmacologic concentrations affects cytokine expression and induces apoptosis of human peripheral blood mononuclear cells

被引:75
作者
Chang, KL [1 ]
Hung, TC
Hsieh, BS
Chen, YH
Chen, TF
Cheng, HL
机构
[1] Kaohsiung Med Univ, Coll Med, Fac Med, Dept Biochem, Kaohsiung, Taiwan
[2] Wu Kun Che Gynecol Obstet & Pediat Hosp, Kaohsiung, Taiwan
[3] Kaohsiung Med Univ, Chung Ho Mem Hosp, Dept Obstet & Gynecol, Kaohsiung, Taiwan
关键词
zinc; cytokines; apoptosis; caspase-3; nuclear factor-kappa B; c-fos;
D O I
10.1016/j.nut.2005.11.009
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
Objective: The present study examined the effect of zinc at concentrations of the apoptotic signaling pathway and immune function of peripheral blood mononuclear cells (PBMCs). Methods: PBMCs from healthy subjects were treated in vitro with various zinc concentrations to imitate different serum statuses of physiologic (2 to 15 mu M) and pharmacologic (15 to 100 mu M) concentrations to higher than 100 mu M and analyzed their expressions of cytokines and apoptotically related factors. Results: Although a normal physiologic concentration of zinc had no effect on immunologic function or apoptosis of PBMCs, a pharmacologic concentration (100 mu M) or higher affected both functions. Zinc decreased cell proliferation at concentrations higher than 100 mu M and stimulated cytokine expression at concentrations of at least 100 mu M. Further, at concentrations of at least 100 mu M, apoptosis was induced, and expressions of caspase-3 and proapoptotic genes, including Fas (FasL) and c-fos, which trigger apoptosis through receptor-mediated extrinsic and mitochondrion-mediated apoptotic pathways, respectively, were increased. At concentrations at least 300 mu M, expressions of antiapoptotic factors nuclear factor-kappa B, Bcl-2, and Bcl-X-L were markedly decreased. Conclusions: Zinc stimulates cytokine expression and induces apoptosis of PBMCs from healthy subjects only at concentrations equal to or greater than the serum pharmacologic range. Receptor-mediated extrinsic and mitochondrial-mediated intrinsic pathways are involved in this zinc-induced apoptosis. (C) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码:465 / 474
页数:10
相关论文
共 50 条
  • [31] Selected scorpion toxin exposures induce cytokine release in human peripheral blood mononuclear cells
    Corzo, Gerardo
    Espino-Solis, Gerardo Pavel
    TOXICON, 2017, 127 : 56 - 62
  • [32] Dynamics of cytokine production in human peripheral blood mononuclear cells stimulated by LPS or infected by Borrelia
    Jansky, L
    Reymanová, P
    Kopecky, J
    PHYSIOLOGICAL RESEARCH, 2003, 52 (05) : 593 - 598
  • [33] Growth of Chlamydia pneumoniae in cultured human peripheral blood mononuclear cells and induction of a cytokine response
    KaukorantaTolvanen, SSE
    Teppo, AM
    Laitinen, K
    Saikku, P
    Linnavuori, K
    Leinonen, M
    MICROBIAL PATHOGENESIS, 1996, 21 (03) : 215 - 221
  • [34] Cytokine secretion from human peripheral blood mononuclear cells cultured in vitro with metal particles
    Cachinho, Sandra C. P.
    Pu, Fanrong
    Hunt, John A.
    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2013, 101 (04) : 1201 - 1209
  • [35] Spontaneous apoptosis in peripheral blood mononuclear cells of leprosy patients: role of cytokines
    Gupta, A
    Sharma, VK
    Vohra, H
    Ganguly, NK
    FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY, 1999, 24 (01): : 49 - 55
  • [36] Proliferation and Apoptosis of Peripheral Blood Mononuclear Cells in Patients with Oral Lichen Planus
    Shan, Jing
    Ma, Jiang-Min
    Wang, Ran
    Liu, Qing-Lan
    Fan, Yuan
    INFLAMMATION, 2013, 36 (02) : 419 - 425
  • [37] Temporal cytokine profiling of Francisella tularensis-infected human peripheral blood mononuclear cells
    Paranavitana, Chrysanthi
    Pittman, Phillip
    Velauthapillai, Mahendran
    DaSilva, Luis
    JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION, 2008, 41 (03) : 192 - 199
  • [38] Sepsis induces increased apoptosis in lamina propria mononuclear cells which is associated with altered cytokine gene expression
    Chung, SC
    Xu, YX
    Chaudry, IH
    Ayala, A
    JOURNAL OF SURGICAL RESEARCH, 1998, 77 (01) : 63 - 70
  • [39] Heparin induces apoptosis in human peripheral blood neutrophils
    Manaster, J
    Chezar, J
    ShurtzSwirski, R
    Shapiro, G
    Tendler, Y
    Kristal, B
    Shasha, SM
    Sela, S
    BRITISH JOURNAL OF HAEMATOLOGY, 1996, 94 (01) : 48 - 52
  • [40] LPS induces apoptosis of peripheral blood dendritic cells
    Kubicka-Sierszen, Agata
    Michalak, Anna
    Kowalski, Marek L.
    Giszterowicz, Magdalena
    Grzegorczyk, Janina L.
    ALERGIA ASTMA IMMUNOLOGIA, 2016, 21 (02): : 121 - 126