Chromatographic Fingerprint Analysis of Semen Ziziphi spinosae by HPLC-DAD Method

被引：13

作者：

Zeng, Wenzhi

Bai, Yanjing

Zhang, Qingying ^{[1
]}

Zhao, Yuying

机构：

[1] Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Dept Nat Med, Beijing, Peoples R China

来源：

ANALYTICAL LETTERS | 2009年 / 42卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Chromatographic fingerprint; HPLC; Semen Ziziphi spinosae; Ziziphus; Ziziphus jujuba var; spinosa;

D O I：

10.1080/00032710802585642

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Reliable, reproducible and valid fingerprint analysis methods using high-performance liquid chromatography-photodiode array detection (HPLC-DAD) for characteristic bioactive flavonoids and saponins of Semen Ziziphi spinosae (SZS) were developed and validated. HPLC separation of the chemical constituents of SZS was performed on an YMC-PACK ODS-A RP-18 column and detected at 270 and 204nm for flavonoids and saponins, respectively. A mobile phase consisted of acetonitrile and 0.1% phosphoric acid aqueous solution was used with linear gradient elution. Using spinosin and jujuboside B as the reference markers of flavonoids and saponins respectively, 9 common fingerprint peaks of flavonoids and 10 common fingerprint peaks of saponins were specified based on the fingerprint analysis of 10 batches of SZS from different regions in China. The fingerprint analysis methods developed are reliable, reproducible and valid, and might be used as a more convenient approach for the species identification and quality monitoring and assessment of SZS.

引用

页码：205 / 215

页数：11

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