Upconversion fluorescent strip sensor for rapid determination of Vibrio anguillarum

被引：83

作者：

Zhao, Peng ^{[1
]}

Wu, Yuanyuan ^{[2
]}

Zhu, Yihua ^{[1
]}

Yang, Xiaoling ^{[1
]}

Jiang, Xin ^{[1
]}

Xiao, Jingfan ^{[2
]}

Zhang, Yuanxing ^{[2
]}

Li, Chunzhong ^{[1
]}

机构：

[1] E China Univ Sci & Technol, Key Lab Ultrafine Mat, Minist Educ, Sch Mat Sci & Engn, Shanghai 200237, Peoples R China

[2] E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

来源：

NANOSCALE | 2014年 / 6卷 / 07期

基金：

中国国家自然科学基金;

关键词：

CONVERTING PHOSPHOR REPORTERS; LATERAL-FLOW; ASSAY; NANOCRYSTALS; PHASE; NANOCOMPOSITES; IDENTIFICATION; IMMUNOASSAY; LUMINESCENT; STRATEGY;

D O I：

10.1039/c3nr06549a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Here, we report a simple and ultrasensitive upconversion fluorescent strip sensor based on NaYF4:Yb, Er nanoparticles (NPs) and the lateral flow immunochromatographic assay (LFIA). Carboxyl-modified b-NaYF4:Yb, Er NPs were successfully synthesized by a facile one-pot solvothermal approach, upon further coupling with monoclonal antibody, the resultant UCNPs-antibody conjugates probes were used in LFIA and served as signal vehicles for the fluorescent reporters. V. anguillarum was used as a model analyte to demonstrate the use of this strip sensor. The limit of the detection for the fluorescent strip was determined as 10(2) CFU mL(-1), which is 100 times lower than those displayed by enzyme-linked immunosorbent assays, while the time needed for the detection was only 15 min. Furthermore, no cross-reaction with other eight pathogens was found, indicating the good specificity of the strip. This developed LFIA would offer the potential as a useful tool for the quantification of pathogens analysis in the future.

引用

页码：3804 / 3809

页数：6

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