Novel Endotoxin Assay by Laser Light-Scattering Particle-Counting Method

被引：6

作者：

Mitsumoto, Kotaro ^{[1
]}

Yabusaki, Katsumi ^{[1
]}

Kobayashi, Koji ^{[1
]}

Shirasawa, Yoshiaki ^{[1
]}

Obata, Toru ^{[2
]}

机构：

[1] Kowa Co Ltd, Elect & Opt Res Labs, Tokyo 1820021, Japan

[2] Jikei Univ, Sch Med, Inst DNA Med, Dept Mol Cell Biol, Tokyo, Japan

来源：

JOURNAL OF CLINICAL LABORATORY ANALYSIS | 2009年 / 23卷 / 02期

关键词：

Limulus test; amebocyte lysate; light scattering; LIMULUS AMEBOCYTE LYSATE; TURBIDIMETRIC KINETIC ASSAY; CHROMOGENIC SUBSTRATE; SYSTEM; BLOOD; POLYPHEMUS; INHIBITOR;

D O I：

10.1002/jcla.20300

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Exposure of Limulus amoebocyte lysate to endotoxin under stirring produced light-reflective particles that appeared to be coagulin polymers. A laser light-scattering particle counter, the PA-200, detected these particles sensitively. The PA-200 detected endotoxin at a concentration as low as 0.00015 EU/ml in 71 min, whereas the minimum endotoxin concentration measured by a turbidimeter, ET-2000, was 0.0005 EU/ml in 138min. Moreover, PA-200 was much less affected by the presence of colored substances and refractive materials than was ET-2000. We propose that the high sensitivity, speed, and high interference tolerance of the laser light-scattering particle-counting method make it more useful than the widely used turbidimetric method for quantitative endotoxin assay. J. Clin. Lab. Anal. 23:117-124 (2009). 2009 Wiley-Liss, Inc.

引用

页码：117 / 124

页数：8

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