Evidence for the surface-diffusion mechanism of solution crystallization from molecular-level observations with ferritin

被引:32
作者
Chen, K [1 ]
Vekilov, PG
机构
[1] Univ Alabama, Ctr Micrograv & Mat Res, Huntsville, AL 35899 USA
[2] Univ Houston, Dept Chem Engn, Houston, TX 77204 USA
来源
PHYSICAL REVIEW E | 2002年 / 66卷 / 02期
关键词
D O I
10.1103/PhysRevE.66.021606
中图分类号
O35 [流体力学]; O53 [等离子体物理学];
学科分类号
070204 ; 080103 ; 080704 ;
摘要
We employ atomic force microscopy to monitor in situ, in real time, the molecular processes of crystallization of ferritin, a protein that has an inorganic single-crystalline core that can be varied. We determine the statistics of molecular attachment and detachment at the growth sites and find that the ratio of the fluxes in and out of the kinks is significantly lower than expected, assuming direct incorporation of the molecules from the solution. Determinations of the energy barrier for incorporation yield similar to30 kJ mol-1, significantly higher than expected for this mechanism. We conclude that attachment of molecules occurs via the surface adsorption layer. The surface coverage resulting from this mechanism is similar to0.9, suggesting a growth mode different from the classical surface diffusion mechanism.
引用
收藏
页码:1 / 021606
页数:5
相关论文
共 44 条
  • [1] THE MEANDERING OF STEPS AND THE TERRACE WIDTH DISTRIBUTION ON CLEAN SI(111) - AN INSITU EXPERIMENT USING REFLECTION ELECTRON-MICROSCOPY
    ALFONSO, C
    BERMOND, JM
    HEYRAUD, JC
    METOIS, JJ
    [J]. SURFACE SCIENCE, 1992, 262 (03) : 371 - 381
  • [2] THE INFLUENCE OF STEP STEP INTERACTIONS ON STEP WANDERING
    BARTELT, NC
    EINSTEIN, TL
    WILLIAMS, ED
    [J]. SURFACE SCIENCE, 1990, 240 (1-3) : L591 - L598
  • [3] BAUSER E, 1984, HDB CRYSTAL GROWTH B, V3, P879
  • [4] Bennema P., 1967, J CRYST GROWTH, V1, P278, DOI DOI 10.1016/0022-0248(67)90034-6
  • [5] RATE OF PROPAGATION OF GROWTH LAYERS ON CUBIC CRYSTAL FACES IN ELECTROCRYSTALLIZATION OF SILVER
    BOSTANOV, V
    STAIKOV, G
    ROE, DK
    [J]. JOURNAL OF THE ELECTROCHEMICAL SOCIETY, 1975, 122 (10) : 1301 - 1305
  • [6] Electron microscopic observations on protein crystallization: adsorption layers, aggregates and crystal defects
    Braun, N
    Tack, J
    Fischer, M
    Bacher, A
    Bachmann, L
    Weinkauf, S
    [J]. JOURNAL OF CRYSTAL GROWTH, 2000, 212 (1-2) : 270 - 282
  • [7] THE GROWTH OF CRYSTALS AND THE EQUILIBRIUM STRUCTURE OF THEIR SURFACES
    BURTON, WK
    CABRERA, N
    FRANK, FC
    [J]. PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES A-MATHEMATICAL AND PHYSICAL SCIENCES, 1951, 243 (866) : 299 - 358
  • [8] Chernov A.A., 1961, Sov. Phys. Usp, V4, DOI [10.1070/PU1961v004n01ABEH003328, DOI 10.1070/PU1961V004N01ABEH003328]
  • [9] Chernov A. A., 1995, SCI TECHNOLOGY CRYST, P67
  • [10] Chernov A.A., 1984, Modern Crystallography III: Crystal Growth