Localization of the HIV-1 gp120 conformational epitope recognized by virus-neutralizing monoclonal antibodies 2G12

被引：5

作者：

Tumanova, OY ^{[1
]}

Kuvshinov, VN

Il'ichev, AA

Nekrasov, BG

Ivanisenko, VA

Kozlov, AP

Sandakhchiev, LS

机构：

[1] VECTOR State Res Ctr Virol & Biotechnol, Inst Bioengn, Koltsov 630559, Novosibirsk Reg, Russia

[2] Biomed Ctr, Inst Highly Purified Preparat, St Petersburg 197110, Russia

来源：

MOLECULAR BIOLOGY | 2002年 / 36卷 / 04期

关键词：

HIV-1; gp120; virus-neutralizing monoclonal antibody 2G12; conformational epitope; localization; phage peptide library;

D O I：

10.1023/A:1019804511163

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A phage peptide library was used to select peptides interacting with virus-neutralizing monoclonal antibodies (mAb) 2G12 which recognize a discontinuous surface epitope of HIV-1 gp120. With the published X-ray data, gp 120 regions involved in the antigenic determinant were predicted. Binding with mAb 2G12 was ascribed to Thr297, Phe383, Tyr384, Arg419, Ile240, Thr415, Leu416, Pro417, Lys421, and Trp112. Though distant in the gp 120 sequence, these residues are close in space and form the 2G12 epitope on the gp 120 surface.

引用

页码：517 / 521

页数：5

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