Unraveling altered RNA metabolism in pancreatic cancer cells by liquid-chromatography coupling to ion mobility mass spectrometry

被引：10

作者：

Lagies, Simon ^{[1
,2
,3
]}

Schlimpert, Manuel ^{[1
,2
,3
]}

Braun, Lukas M. ^{[1
,4
]}

Kather, Michel ^{[1
,5
,6
]}

Plagge, Johannes ^{[1
]}

Erbes, Thalia ^{[7
,8
]}

Wittel, Uwe A. ^{[4
]}

Kammerer, Bernd ^{[1
,9
]}

机构：

[1] Albert Ludwigs Univ Freiburg, Ctr Biol Syst Anal ZBSA, Habsburgerstr 49, D-79104 Freiburg, Germany

[2] Albert Ludwigs Univ Freiburg, Inst Biol 2, Schanzlestr 1, D-79104 Freiburg, Germany

[3] Albert Ludwigs Univ Freiburg, Spemann Grad Sch Biol & Med, Albertstr 19A, D-79104 Freiburg, Germany

[4] Univ Freiburg Med Ctr, Dept Gen & Visceral Surg, Hugstetter Str 55, D-79106 Freiburg, Germany

[5] Albert Ludwigs Univ Freiburg, Fac Chem & Pharm, Hebelstr 27, D-79104 Freiburg, Germany

[6] Univ Freiburg, Hermann Staudinger Grad Sch, Hebelstr 27, D-79104 Freiburg, Germany

[7] Univ Freiburg, Fac Med, Dept Gynecol & Obstet, Hugstetter St 55, D-79106 Freiburg, Germany

[8] Univ Freiburg, Med Ctr, Hugstetter St 55, D-79106 Freiburg, Germany

[9] Univ Freiburg, BIOSS Ctr Biol Signalling Studies, Schanzlestr 16, D-79104 Freiburg, Germany

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2019年 / 411卷 / 24期

关键词：

Modified nucleosides; Ion mobility mass spectrometry; TWIMS; Pancreatic cancer; Biomarker; MODIFIED NUCLEOSIDES; URINARY NUCLEOSIDES; K-RAS; ADENOCARCINOMA; CARCINOMA; P53; RIBONUCLEOTIDE; IDENTIFICATION; BIOMARKERS; SEPARATION;

D O I：

10.1007/s00216-019-01814-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Ion mobility coupling to mass spectrometry facilitates enhanced identification certitude. Further coupling to liquid chromatography results in multi-dimensional analytical methods, especially suitable for complex matrices with structurally similar compounds. Modified nucleosides represent a large group of very similar members linked to aberrant proliferation. Besides basal production under physiological conditions, they are increasingly excreted by transformed cells and subsequently discussed as putative biomarkers for various cancer types. Here, we report a method for modified nucleosides covering 37 species. We determined collisional cross-sections with high reproducibility from pure analytical standards. For sample purification, we applied an optimized phenylboronic acid solid-phase extraction on media obtained from four different pancreatic cancer cell lines. Our analysis could discriminate different subtypes of pancreatic cancer cell lines. Importantly, they could clearly be separated from a pancreatic control cell line as well as blank medium. m1A, m27G, and Asm were the most important features discriminating cancer cell lines derived from well-differentiated and poorly differentiated cancers. Eventually, we suggest the analytical method reported here for future tumor-marker identification studies.

引用

页码：6319 / 6328

页数：10