Evaluation of cell numbers and viability of Saccharomyces cerevisiae by different counting methods

被引：20
|
作者：
Fiala, J
Lloyd, DR
Rychtera, M
Kent, CA [1 ]
Al-Rubeai, M
机构：
[1] Univ Birmingham, Sch Chem Engn, Ctr Bioproc Engn, Birmingham B15 2TT, W Midlands, England
[2] Inst Chem Technol, Dept Fermentat Chem & Bioengn, Prague 16628 6, Czech Republic
来源：
BIOTECHNOLOGY TECHNIQUES | 1999年 / 13卷 / 11期
基金：
英国生物技术与生命科学研究理事会;
关键词：
cell number; Coulter principle; flow cytometry; viability; yeast;
D O I：
10.1023/A:1008974419342
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Four counting methods (two flow cytometry, one Coulter principle, one microscopic) are compared for measuring cell density and viability of batch-grown yeast. All gave adequate precision in measuring total cell density with no systematic difference between methods. However, the promise of flow cytometry as a rapid means of determining both quantity and quality of a cell population is shown.
引用
收藏
页码：787 / 795
页数：9
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