Highly sensitive determination of copper in HeLa cell using capillary electrophoresis combined with a simple cell extraction treatment

被引：12

作者：

Meng, Lingchen ^{[1
]}

Fang, Ziyuan ^{[1
]}

Lin, Jian ^{[1
]}

Li, Meixian ^{[1
]}

Zhu, Zhiwei ^{[1
]}

机构：

[1] Peking Univ, Beijing Natl Lab Mol Sci, Coll Chem & Mol Engn, Beijing 100871, Peoples R China

来源：

TALANTA | 2014年 / 121卷

基金：

中国国家自然科学基金;

关键词：

Capillary electrophoresis; Copper; EDTA; HeLa cells; PLASMA-MASS SPECTROMETRY; ATOMIC-ABSORPTION-SPECTROMETRY; METAL-IONS; EMISSION-SPECTROMETRY; FLUORESCENCE; SEPARATION; INJECTION; SAMPLES; CHROMIUM(III); SPECIATION;

D O I：

10.1016/j.talanta.2014.01.009

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new separation system of capillary electrophoresis (CE1) for the highly sensitive determination of copper was established by using ethylenediaminetetraacetic acid (EDTA) as a complexing agent and employing cetyltrimethylammonium chloride (CTAC) as a capillary inner wall modifier. Benefitted from the combination of field-enhanced sample injection (FESI) method, a limit of detection (LOD) of 2.7 nM was obtained, which was much lower than that of the conventional methods. This made it possible to determine trace copper in HeLa cell only by a simple cell extraction (CE2) treatment. Two copper-extraction methods acid-hydrolysis and freeze-thaw were compared. Limited by the requirement of low ion strength in FESI, only the extract using freeze-thaw could be successfully applied in the determination. The effectiveness assessment of this CE2-FESI method was adopted by inductively coupled plasma-atomic emission spectrometry (ICP-AES) as a gold standard. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：205 / 209

页数：5

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