Isolation and identification of unsaturated fatty acid methyl esters from marine micro-algae

被引：21

作者：

Viron, C

Saunois, A

André, P

Perly, B

Lafosse, M

机构：

[1] Univ Orleans, CNRS, UPRES A 6005, Inst Chim Organ & Analyt,UFR Sci, F-45067 Orleans 2, France

[2] Parfums Christian Dior, Lab Subst Nat, F-45804 St Jean De Braye, France

[3] CEN, Serv Chim Mol, F-91191 Gif Sur Yvette, France

来源：

ANALYTICA CHIMICA ACTA | 2000年 / 409卷 / 1-2期

关键词：

porous graphitic carbon phase; semi-preparative liquid chromatography; hexadecatrienoic acid methyl ester; hexadecadienoic acid methyl ester; micro-alga;

D O I：

10.1016/S0003-2670(99)00857-0

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In order to study the biological activity of a micro-algae extract rich in fatty acids, the purification and structural identification of two hexadecadienoic methyl esters (16 : 2) and one hexadecatrienoic methyl ester (16 : 3) were performed. The purification of these molecules was carried out by liquid chromatography using a porous graphitic carbon phase. Determination of the chain length, degree and position of unsaturations was achieved by gas chromatography/electron impact mass spectrometer on dimethyloxazoline derivatives. With regard to the double bonds cis or trans isomery was determined by Fourier transform infrared spectroscopy Structures were confirmed by H-1 and C-13 nuclear magnetic resonance. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：257 / 266

页数：10

共 14 条

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[2]

BARNATHAN G, 1993, THESIS NANTES

[3] LOCATION OF DOUBLE-BONDS IN POLYUNSATURATED FATTY-ACIDS BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY AFTER 4,4-DIMETHYLOXAZOLINE DERIVATIZATION [J].