Geiparvarin Inhibits OS Metastasis through Upregulation of ANGPTL4 Expression by Inhibiting miRNA-3912-3p Expression

被引：5

作者：

Jiang, Fuling ^{[1
]}

Wang, Guang-Jie ^{[2
]}

Huang, Ping ^{[3
]}

Chen, Shu ^{[4
]}

Xiao, He ^{[4
]}

Zhang, Liang ^{[4
]}

Zou, Hua ^{[4
]}

机构：

[1] Army Med Univ, Daping Hosp, Ctr Orthoped, Dept Spine Surg, Chongqing 400042, Peoples R China

[2] Gen Hosp Western Theater Command, Dept Oncol, Chengdu 610083, Sichuan, Peoples R China

[3] Chongqing Univ Canc Hosp, Dept Radiat Oncol, Chongqing 400033, Peoples R China

[4] Army Med Univ, Daping Hosp, Canc Ctr, Chongqing 400042, Peoples R China

来源：

EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE | 2022年 / 2022卷

关键词：

OSTEOSARCOMA; GROWTH; MICRORNAS;

D O I：

10.1155/2022/4663684

中图分类号：

R [医药、卫生];

学科分类号：

10 ;

摘要：

Background. Geiparvarin (GN) is a natural compound with anticancer activity. However, the effect of GN on osteosarcoma (OS) and the anticancer mechanism of GN are still unclear. Methods. Cell viability was measured by MTT assay. Invasion and migration were measured by transwell assay. The miRNAs, genes, and signaling pathways affected by GN were confirmed by whole-genome sequencing and bioinformatics analysis. The expression level of mRNA and protein was measured by qRT-PCR and western blot. Animal experiment was performed for confirming the GN anticancer effect and side effect in vivo. Results. Our results show that GN significantly inhibits OS cell growth and metastasis in vitro. In vivo experiment also showed that GN dramatically suppressed OS lung metastasis and no side effects were found. GN treatment inhibited OS metastasis through upregulating the ANGPTL4 expression. In addition, GN inhibited the expression of miR-3912-3p, which targets ANGPTL4. Conclusion. Our data clearly indicate that GN is a candidate drug for OS treatment, and GN plays its role through miR-3912-3p/ANGPTL4 in OS.

引用

页数：8

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