MicroRNA-mediated interacting circuits predict hypoxia and inhibited osteogenesis of stem cells, and dysregulated angiogenesis are involved in osteonecrosis of the femoral head

被引:18
作者
Kao, Gour-Shenq [1 ,2 ]
Tu, Yuan-Kun [3 ]
Sung, Pei-Hsun [4 ]
Wang, Feng-Sheng [2 ,5 ]
Lu, Yu-Der [1 ]
Wu, Chen-Ta [1 ]
Lin, Rio L. C. [1 ,2 ]
Yip, Hon-Kan [4 ]
Lee, Mel S. [1 ]
机构
[1] Kaohsiung Chang Gung Mem Hosp, Dept Orthoped Surg, 123 Ta Pei Rd, Kaohsiung 833, Taiwan
[2] Kaohsiung Chang Gung Mem Hosp, Dept Med Res, 123 Ta Pei Rd, Kaohsiung 833, Taiwan
[3] E Da Hosp, Dept Orthoped Surg, Kaohsiung, Taiwan
[4] Kaohisung Chang Gung Mem Hosp, Div Cardiol, Dept Internal Med, 123 Ta Pei Rd, Kaohsiung 833, Taiwan
[5] Chang Gung Univ, Coll Med, Grad Inst Clin Med Sci, Taoyuan, Taiwan
关键词
Osteonecrosis; MicroRNA; Microarray; Bioinformatics; DIFFERENTIALLY EXPRESSED GENES; CIRCULATING MICRORNAS; HYPOFIBRINOLYSIS; MECHANISM; CLUSTER; BONE; P53;
D O I
10.1007/s00264-018-3895-x
中图分类号
R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学(修复外科学)];
学科分类号
摘要
Purpose MicroRNAs (miRNAs) are associated with various pathologic conditions and can serve as diagnostic or therapeutic biomarkers. This study tried to identify the differentially expressed miRNAs to predict the possible pathomechanisms involved in osteonecrosis of the femoral head (ONFH). Methods We compared the peripheral blood miRNAs in 46 patients with ONFH and 85 healthy controls by microarray and droplet digital polymerase chain reaction (ddPCR). Putative interacted networks between the differentially responded miRNAs were analyzed by web-based bioinformatics prediction tools. Results Microarray identified 51 differentially expressed miRNAs with at least twofold change (upregulation in 34 and downregulation in 17). and the results were validated by ddPCR using six selected miRNAs. Bioinformatics genetic network analysis focusing on the six miRNAs found the upregulated miR-18a and miR-19a are associated with angiogenesis after induction of ischemia; the upregulated miR-138-1 can inhibit osteogenic differentiation of mesenchymal stem cells; the most targeted genes, p53 and SERBP1, are associated with hypoxia and hypofibrinolysis. Conclusions This study combined the miRNA analysis with the bioinformatics and predicts that hypoxia, inhibited osteogenesis of stem cells, and dysregulated angiogenesis might be orchestrated through the miRNA interacting circuits in the pathogenesis of ONFH.
引用
收藏
页码:1605 / 1614
页数:10
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