Sensitivity of Neurospora crassa to a Marine-Derived Aspergillus tubingensis Anhydride Exhibiting Antifungal Activity That Is Mediated by the MAS1 Protein

被引：30

作者：

Koch, Liat ^{[1
]}

Lodin, Anat ^{[2
]}

Herold, Inbal ^{[1
]}

Ilan, Micha ^{[3
]}

Carmeli, Shmuel ^{[2
]}

Yarden, Oded ^{[1
]}

机构：

[1] Hebrew Univ Jerusalem, RH Smith Fac Agr Food & Environm, Dept Plant Pathol & Microbiol, IL-76100 Rehovot, Israel

[2] Tel Aviv Univ, Raymond & Beverly Sackler Fac Exact Sci, Sch Chem, IL-69978 Tel Aviv, Israel

[3] Tel Aviv Univ, George S Wise Fac Life Sci, Dept Zool, IL-69978 Tel Aviv, Israel

来源：

MARINE DRUGS | 2014年 / 12卷 / 09期

基金：

以色列科学基金会;

关键词：

natural products; antifungal; Aspergillus; tubingensis; Neurospora crassa; cell wall; chitin synthase; CHITIN SYNTHASE; BIOACTIVE METABOLITES; GENOME SEQUENCE; GENE-EXPRESSION; SPONGES; FUNGUS; MICROORGANISMS; LOCALIZATION; FUMIGATUS; KINASE;

D O I：

10.3390/md12094713

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The fungus Aspergillustubingensis (strain OY907) was isolated from the Mediterranean marine sponge Ircinia variabilis. Extracellular extracts produced by this strain were found to inhibit the growth of several fungi. Among the secreted extract components, a novel anhydride metabolite, tubingenoic anhydride A (1) as well as the known 2-carboxymethyl-3-hexylmaleic acid anhydride, asperic acid, and campyrone A and C were purified and their structure elucidated. Compound 1 and 2-carboxymethyl-3-hexylmaleic acid anhydride inhibited Neurospora crassa growth (MIC = 330 and 207 M, respectively) and affected hyphal morphology. We produced a N. crassa mutant exhibiting tolerance to 1 and found that a yet-uncharacterized gene, designated mas-1, whose product is a cytosolic protein, confers sensitivity to this compound. The mas-1 strain showed increased tolerance to sublethal concentrations of the chitin synthase inhibitor polyoxin D, when compared to the wild type. In addition, the expression of chitin synthase genes was highly elevated in the mas-1 strain, suggesting the gene product is involved in cell wall biosynthesis and the novel anhydride interferes with its function.

引用

页码：4713 / 4731

页数：19