Magnetic assisted fluorescence immunoassay for sensitive chloramphenicol detection using carbon dots@CaCO3 nanocomposites

被引：0

作者：

Dong, Baolei ^{[1
]}

Li, Hongfang ^{[1
]}

Sun, Jiefang ^{[2
]}

Li, Yuan ^{[1
]}

Mari, Ghulam Mujtaba ^{[1
]}

Yu, Xuezhi ^{[1
]}

Yu, Wenbo ^{[1
]}

Wen, Kai ^{[1
]}

Shen, Jianzhong ^{[1
]}

Wang, Zhanhui ^{[1
]}

机构：

[1] China Agr Univ, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Beijing Lab Food Qual & Safety, Beijing 100193, Peoples R China

[2] Beijing Res Ctr Prevent Med, Beijing Key Lab Diagnost & Traceabil Food Poisoni, Beijing 100013, Peoples R China

来源：

JOURNAL OF HAZARDOUS MATERIALS | 2021年 / 402卷

基金：

中国国家自然科学基金;

关键词：

Carbon dots; Fluorescent immunoassay; Chloramphenicol; Nano calcium carbonate; Magnetic nanoparticles; QUANTUM DOTS; SOLVOTHERMAL SYNTHESIS; ANTIBIOTIC-RESIDUES; CALCIUM-CARBONATE; NANOPARTICLES; PARTICLES; BOVINE; FOOD;

D O I：

10.1016/j.hazmat.2020.123942

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Analytical methods with high sensitivities and short assay times are urgently required for the screening of "zero tolerance" hazardous substances in food. Herein, we propose a fluorescent immunoassay for the highly sensitive and rapid analysis of chloramphenicol (CAP) based on carbon dots (CDs)-encapsulated CaCO3 nanospheres and magnetic nanoparticles (MNPs). The fluorescent immunoprobes were prepared by coupling the anti-CAP antibodies to carboxymethyl cellulose-functional CDs@CaCO3 nanospheres. Chitosan-modified MNPs with "core- shell" structures were prepared and then conjugated to the CAP hapten, acting as the nano-carrier and interface for the immunoreaction. With the assistance of MNPs, the established fluorescent immunoassay achieved the sensitive detection of CAP in chicken with a limit of detection of 0.03 mu g kg(-1) and recoveries ranging from 83.7%-105.0%. The analysis results of the fluorescent immunoassay were evaluated by the enzyme-linked immunosorbent assay, having a correlation coefficient of 0.981. Our work provides a rapid, facile, and reliable strategy for the highly sensitive analysis of food contaminants based on "green" fluorescent nanoprobes.

引用

页数：9