Magnetic assisted fluorescence immunoassay for sensitive chloramphenicol detection using carbon dots@CaCO3 nanocomposites

被引:0
|
作者
Dong, Baolei [1 ]
Li, Hongfang [1 ]
Sun, Jiefang [2 ]
Li, Yuan [1 ]
Mari, Ghulam Mujtaba [1 ]
Yu, Xuezhi [1 ]
Yu, Wenbo [1 ]
Wen, Kai [1 ]
Shen, Jianzhong [1 ]
Wang, Zhanhui [1 ]
机构
[1] China Agr Univ, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Beijing Lab Food Qual & Safety, Beijing 100193, Peoples R China
[2] Beijing Res Ctr Prevent Med, Beijing Key Lab Diagnost & Traceabil Food Poisoni, Beijing 100013, Peoples R China
基金
中国国家自然科学基金;
关键词
Carbon dots; Fluorescent immunoassay; Chloramphenicol; Nano calcium carbonate; Magnetic nanoparticles; QUANTUM DOTS; SOLVOTHERMAL SYNTHESIS; ANTIBIOTIC-RESIDUES; CALCIUM-CARBONATE; NANOPARTICLES; PARTICLES; BOVINE; FOOD;
D O I
10.1016/j.hazmat.2020.123942
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Analytical methods with high sensitivities and short assay times are urgently required for the screening of "zero tolerance" hazardous substances in food. Herein, we propose a fluorescent immunoassay for the highly sensitive and rapid analysis of chloramphenicol (CAP) based on carbon dots (CDs)-encapsulated CaCO3 nanospheres and magnetic nanoparticles (MNPs). The fluorescent immunoprobes were prepared by coupling the anti-CAP antibodies to carboxymethyl cellulose-functional CDs@CaCO3 nanospheres. Chitosan-modified MNPs with "core- shell" structures were prepared and then conjugated to the CAP hapten, acting as the nano-carrier and interface for the immunoreaction. With the assistance of MNPs, the established fluorescent immunoassay achieved the sensitive detection of CAP in chicken with a limit of detection of 0.03 mu g kg(-1) and recoveries ranging from 83.7%-105.0%. The analysis results of the fluorescent immunoassay were evaluated by the enzyme-linked immunosorbent assay, having a correlation coefficient of 0.981. Our work provides a rapid, facile, and reliable strategy for the highly sensitive analysis of food contaminants based on "green" fluorescent nanoprobes.
引用
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页数:9
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