Helicobacter pylori (H. pylori) has become accepted as a human pathogen for the development of gastritis and gastroduodenal ulcer. To develop a simple rat model of chronic H. pylori infection, male Sprague-Dawley rats were pretreated with streptomycin suspended in tap water (5 mg/mL) for 3 d. The rats were inoculated by gavage at 1 mL/rat with H. pylori suspension (5 x 10(8)-5 x 10(10) CFU/mL) twice daily at an interval of 4 h for three consecutive days. Two weeks after inoculation, rats were sacrificed and the stomachs were removed. Antral biopsies were performed for urease test and the stomachs were taken for histopathology. Successful H. pylori inoculation was defined as a positive urease test and histopathology. We reported a 69.8%-83.0% success rate for H. pylori infection using the urease test, and hematoxylin and eosin staining confirmed the results. Histopathological analysis detected bacteria along the mucous lining of the surface epithelium and crypt lumen and demonstrated mild to moderate gastric inflammation in successfully inoculated rats. We developed a simple rat model of chronic H. pylori infection for research into gastric microcirculatory changes and therapy with plant products. (C) 2014 Baishideng Publishing Group Inc. All rights reserved.
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Ludwig Maximilians Univ Munchen, Univ Hosp, Med Dept 2, Munich, Germany
Otto von Guericke Univ, Med Dept Klin Gastroenterol Hepatol & Infectiol, Magdeburg, GermanyLudwig Maximilians Univ Munchen, Univ Hosp, Med Dept 2, Munich, Germany
Malfertheiner, Peter
Camargo, M. Constanza
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NCI, Div Canc Epidemiol & Genet, Rockville, MD USALudwig Maximilians Univ Munchen, Univ Hosp, Med Dept 2, Munich, Germany
Camargo, M. Constanza
El-Omar, Emad
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St George & Sutherland Clin Campuses, Fac Med & Hlth, Microbiome Res Ctr, Sch Clin Med, Sydney, NSW, AustraliaLudwig Maximilians Univ Munchen, Univ Hosp, Med Dept 2, Munich, Germany
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St Antonio Hosp, Azienda ULSS Padova 16, Geriatr Unit, I-35127 Padua, ItalySt Antonio Hosp, Azienda ULSS Padova 16, Geriatr Unit, I-35127 Padua, Italy
Pilotto, Alberto
Franceschi, Marilisa
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ULSS 4 AltoVicentino, Dept Surg, Endoscop Unit, I-36014 Santorso, ItalySt Antonio Hosp, Azienda ULSS Padova 16, Geriatr Unit, I-35127 Padua, Italy
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AI Evdokimov Moscow State Univ Med & Dent, Minist Hlth Russia, Moscow, RussiaAI Evdokimov Moscow State Univ Med & Dent, Minist Hlth Russia, Moscow, Russia
Andreev, D. N.
Kucheryavyi, Yu. A.
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AI Evdokimov Moscow State Univ Med & Dent, Minist Hlth Russia, Moscow, RussiaAI Evdokimov Moscow State Univ Med & Dent, Minist Hlth Russia, Moscow, Russia