Simple animal model of Helicobacter pylori infection

被引:21
|
作者
Werawatganon, Duangporn [1 ]
机构
[1] Chulalongkorn Univ, Fac Med, Dept Physiol, Bangkok 10330, Thailand
关键词
Helicobacter pylori; Rat model; Chronic infection; GASTRIC-MUCOSAL MICROCIRCULATION; PEPTIC-ULCER DISEASE; IN-VIVO; RATS; PATHOGENESIS; EXTRACTS;
D O I
10.3748/wjg.v20.i21.6420
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Helicobacter pylori (H. pylori) has become accepted as a human pathogen for the development of gastritis and gastroduodenal ulcer. To develop a simple rat model of chronic H. pylori infection, male Sprague-Dawley rats were pretreated with streptomycin suspended in tap water (5 mg/mL) for 3 d. The rats were inoculated by gavage at 1 mL/rat with H. pylori suspension (5 x 10(8)-5 x 10(10) CFU/mL) twice daily at an interval of 4 h for three consecutive days. Two weeks after inoculation, rats were sacrificed and the stomachs were removed. Antral biopsies were performed for urease test and the stomachs were taken for histopathology. Successful H. pylori inoculation was defined as a positive urease test and histopathology. We reported a 69.8%-83.0% success rate for H. pylori infection using the urease test, and hematoxylin and eosin staining confirmed the results. Histopathological analysis detected bacteria along the mucous lining of the surface epithelium and crypt lumen and demonstrated mild to moderate gastric inflammation in successfully inoculated rats. We developed a simple rat model of chronic H. pylori infection for research into gastric microcirculatory changes and therapy with plant products. (C) 2014 Baishideng Publishing Group Inc. All rights reserved.
引用
收藏
页码:6420 / 6424
页数:5
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