Androgen rapidly increases dendritic thorns of CA3 neurons in male rat hippocampus

被引：65
作者：
Hatanaka, Yusuke [1 ,3 ]
Mukai, Hideo [1 ,2 ,3 ]
Mitsuhashi, Kenji [1 ,3 ]
Hojo, Yasushi [1 ,2 ,3 ]
Murakami, Gen [1 ,3 ]
Komatsuzaki, Yoshimasa [1 ]
Sato, Rei [1 ,3 ]
Kawato, Suguru [1 ,2 ,3 ]
机构：
[1] Univ Tokyo, Grad Sch Arts & Sci, Dept Biophys & Life Sci, Meguro Ku, Tokyo 1538902, Japan
[2] Univ Tokyo, Japan Sci & Technol Agcy, Core Res Evolut Sci & Technol Project, Tokyo 1538902, Japan
[3] Univ Tokyo, Japan Sci & Technol Agcy, Bioinformat Project, Tokyo 1538902, Japan
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 381卷 / 04期
关键词：
Androgen; DHT; Testosterone; Spine; Thorn; CA3; Hippocampus; Synaptic plasticity; SPINE SYNAPTIC DENSITY; HORMONAL-REGULATION; ESTROGEN SYNTHESIS; PYRAMIDAL NEURONS; GONADAL-STEROIDS; DENTATE GYRUS; MOSSY FIBERS; PLASTICITY; MEMORY; BRAIN;
D O I：
10.1016/j.bbrc.2009.02.130
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Modulation of hippocampal synaptic plasticity by androgen has been attracting much attention. Thorns of thorny excrescences of CA3 hippocampal neurons are post-synaptic regions whose presynaptic partners are mossy fiber terminals. Here we demonstrated rapid effects of dihydrotestosterone (DHT) and testosterone (T) on the density of thorns, by imaging Lucifer Yellow-injected neurons in adult male rat hippocampal slices. The application of 10 nM DHT or T induced rapid increase in the density of thorns within 2 h. The androgen-mediated increase was suppressed by blocking several kinases, such as Erk MAPK p38 MAPK PKC. and CaMKII. On the other hand, PKA, PI3K were not involved in the signaling of thorn-genesis. The increase in the thorn density by androgen was also blocked by the inhibitor of classical androgen receptor. Almost no difference was observed between DHT and T in the effect on the thorn density. We observed that the androgen-induced thorn-genesis is opposite to estrogen-induced thorn-degeneration. (C) 2009 Elsevier Inc. All rights reserved.
引用
收藏
页码：728 / 732
页数：5
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