Unique Epitopes Recognized by Antibodies Induced in Chikungunya Virus-Infected Non-Human Primates: Implications for the Study of Immunopathology and Vaccine Development

被引:43
作者
Kam, Yiu-Wing [1 ]
Lee, Wendy W. L. [1 ,2 ]
Simarmata, Diane [1 ]
Le Grand, Roger [3 ,4 ]
Tolou, Hugues [5 ]
Merits, Andres [6 ]
Roques, Pierre [3 ,4 ]
Ng, Lisa F. P. [1 ,7 ]
机构
[1] Biopolis, Agcy Sci Technol & Res A STAR, Singapore Immunol Network, Singapore, Singapore
[2] Natl Univ Singapore, NUS Grad Sch Integrat Sci & Engn, Singapore 117548, Singapore
[3] CEA, Div ImmunoVirol SIV, Inst Emerging Dis & Innovat Therapies IMETI, Fontenay Aux Roses, France
[4] Univ Paris 11, UMR E1, Orsay, France
[5] Grp Etude Preventol, Villenave Dornon, France
[6] Univ Tartu, Inst Technol, EE-50090 Tartu, Estonia
[7] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Biochem, Singapore 117595, Singapore
关键词
MONOCLONAL-ANTIBODIES; REUNION ISLAND; REPLICATION; REEMERGENCE; GLYCOPROTEIN; CHALLENGE; EPIDEMIC; EAST; MICE;
D O I
10.1371/journal.pone.0095647
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Chikungunya virus (CHIKV) is an Alphavirus that causes chronic and incapacitating arthralgia in humans. Although patient cohort studies have shown the production of CHIKV specific antibodies, the fine specificity of the antibody response against CHIKV is not completely defined. The macaque model of CHIKV infection was established due to limitations of clinical specimens. More importantly, its close relation to humans will allow the study of chronic infection and further identify important CHIKV targets. In this study, serum samples from CHIKV-infected macaques collected at different time-points post infection were used to characterize the antibody production pattern and kinetics. Results revealed that anti-CHIKV antibodies were neutralizing and the E2 glycoprotein, Capsid, nsP1, nsP3 and nsP4 proteins were targets of the anti-CHIKV antibody response in macaques. Furthermore, linear B-cell epitopes recognized by these anti-CHIKV antibodies were identified, and mapped to their structural localization. This characterizes the specificity of anti-CHIKV antibody response in macaques and further demonstrates the importance of the different regions in CHIKV-encoded proteins in the adaptive immune response. Information from this study provides critical knowledge that will aid in the understanding of CHIKV infection and immunity, vaccine design, and pre-clinical efficacy studies.
引用
收藏
页数:11
相关论文
共 55 条
[1]   An epitope of the Semliki Forest virus fusion protein exposed during virus-membrane fusion [J].
Ahn, A ;
Klimjack, MR ;
Chatterjee, PK ;
Kielian, M .
JOURNAL OF VIROLOGY, 1999, 73 (12) :10029-10039
[2]   A Specific Domain of the Chikungunya Virus E2 Protein Regulates Particle Formation in Human Cells: Implications for Alphavirus Vaccine Design [J].
Akahata, Wataru ;
Nabel, Gary J. .
JOURNAL OF VIROLOGY, 2012, 86 (16) :8879-8883
[3]   A virus-like particle vaccine for epidemic Chikungunya virus protects nonhuman primates against infection [J].
Akahata, Wataru ;
Yang, Zhi-Yong ;
Andersen, Hanne ;
Sun, Siyang ;
Holdaway, Heather A. ;
Kong, Wing-Pui ;
Lewis, Mark G. ;
Higgs, Stephen ;
Rossmann, Michael G. ;
Rao, Srinivas ;
Nabel, Gary J. .
NATURE MEDICINE, 2010, 16 (03) :334-U134
[4]  
[Anonymous], J INFECT DI IN PRESS
[5]   Reemergence of Chikungunya Virus in Bo, Sierra Leone [J].
Ansumana, Rashid ;
Jacobsen, Kathryn H. ;
Leski, Tomasz A. ;
Covington, Andrea L. ;
Bangura, Umaru ;
Hodges, Mary H. ;
Lin, Baochuan ;
Bockarie, Alfred S. ;
Lamin, Joseph M. ;
Bockarie, Moses J. ;
Stenger, David A. .
EMERGING INFECTIOUS DISEASES, 2013, 19 (07) :1108-1110
[6]   Chikungunya virus strains, Reunion Island outbreak [J].
Bessaud, Mael ;
Peyrefitte, Christophe N. ;
Pastorino, Boris A. M. ;
Tock, Fabienne ;
Merle, Olivier ;
Colpart, Jean-Jacques ;
Dehecq, Jean-Sebastien ;
Girod, Romain ;
Jaffar-Bandjee, Marie-Christine ;
Glass, Pamela J. ;
Parker, Michael ;
Tolou, Hugues J. ;
Grandadam, Marc .
EMERGING INFECTIOUS DISEASES, 2006, 12 (10) :1604-1606
[7]   A recombinant measles vaccine expressing chikungunya virus-like particles is strongly immunogenic and protects mice from lethal challenge with chikungunya virus [J].
Brandler, Samantha ;
Ruffle, Claude ;
Combredet, Chantal ;
Brault, Jean-Baptiste ;
Najburg, Valerie ;
Prevost, Marie-Christine ;
Habel, Andre ;
Tauber, Erich ;
Despres, Philippe ;
Tangy, Frederic .
VACCINE, 2013, 31 (36) :3718-3725
[8]   Production and characterization of mouse monoclonal antibodies reactive to Chikungunya envelope E2 glycoprotein [J].
Brehin, Anne-Claire ;
Rubrecht, Laetitia ;
Navarro-Sanchez, Martha Erika ;
Marechal, Valerie ;
Frenkiel, Marie-Pascale ;
Lapalud, Priscilla ;
Laurie, Daniel ;
Sall, Amadou Alpha ;
Despres, Philippe .
VIROLOGY, 2008, 371 (01) :185-195
[9]   Comparative Pathogenesis of Epidemic and Enzootic Chikungunya Viruses in a Pregnant Rhesus Macaque Model [J].
Chen, Ching-I ;
Clark, David C. ;
Pesavento, Patricia ;
Lerche, Nicholas W. ;
Luciw, Paul A. ;
Reisen, William K. ;
Brault, Aaron C. .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2010, 83 (06) :1249-1258
[10]   Comparative analysis of the genome sequences and replication profiles of chikungunya virus isolates within the East, Central and South African (ECSA) lineage [J].
Chen, Karen Caiyun ;
Kam, Yiu-Wing ;
Lin, Raymond Tzer Pin ;
Ng, Mary Mah-Lee ;
Ng, Lisa F. P. ;
Chu, Justin Jang Hann .
VIROLOGY JOURNAL, 2013, 10