Human Nop5/Nop58 is a component common to the box C/D small nucleolar ribonucleoproteins

被引：64
作者：
Lyman, SK
Gerace, L
Baserga, SJ
机构：
[1] Yale Univ, Sch Med, Dept Therapeut Radiol, New Haven, CT 06520 USA
[2] Yale Univ, Sch Med, Dept Genet, New Haven, CT 06520 USA
[3] Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA
[4] Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA
来源：
RNA | 1999年 / 5卷 / 12期
关键词：
genomics; nucleolus; ribosome biogenesis; snoRNPs;
D O I：
10.1017/S1355838299991288
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have identified an apparent human homolog of the yeast Nop5/Nop58 protein. hNop5/Nop58 codes for a protein of predicted molecular weight 59.6 kDa and is 46.8% identical to Saccharomyces cerevisiae Nop5/Nop58. Immunofluorescent staining with antibodies against hNop5/Nop58 indicate that it is localized primarily to the nucleolus, and coimmunoprecipitation from nuclear extracts demonstrates that hNop5/Nop58 interacts with the box C/D family of snoRNAs. Thus, hNop5/Nop58 is a common component of the box C/D snoRNPs, and joins fibrillarin as the second such component identified and characterized in metazoans.
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收藏
页码：1597 / 1604
页数：8
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