Anti-influenza virus effects of both live and non-live Lactobacillus acidophilus L-92 accompanied by the activation of innate immunity

被引：66

作者：

Goto, Hiroaki ^{[1
]}

Sagitani, Atsuhiro ^{[1
]}

Ashida, Nobuhisa ^{[2
]}

Kato, Shinji ^{[1
]}

Hirota, Tatsuhiko ^{[1
]}

Shinoda, Tadashi ^{[3
]}

Yamamoto, Naoyuki ^{[3
]}

机构：

[1] Calpis Co Ltd, Microbiol & Fermentat Lab, Chuo Ku, Sagamihara, Kanagawa 2520206, Japan

[2] Calpis Co Ltd, Feed Div, Chuo Ku, Sagamihara, Kanagawa 2520206, Japan

[3] Calpis Co Ltd, Res & Dev Planning Dept, Chuo Ku, Sagamihara, Kanagawa 2520206, Japan

来源：

BRITISH JOURNAL OF NUTRITION | 2013年 / 110卷 / 10期

关键词：

Lactobacillus acidophilus L-92; Innate immunity; Antiviral effects; Natural killer activity; Cytokine profiles; INFLUENZA-A VIRUS; PROTECTS MICE; DOUBLE-BLIND; STRAIN L-92; INFECTION; CELLS; MECHANISMS; SYMPTOMS; EXPRESSION; PROBIOTICS;

D O I：

10.1017/S0007114513001104

中图分类号：

R15 [营养卫生、食品卫生]; TS201 [基础科学];

学科分类号：

100403 ;

摘要：

The antiviral effects of both a live and non-live Lactobacillus acidophilus strain L-92 (L-92) were investigated by oral administration (10 mg/mouse per d) daily for 21 d in a mouse model infected intranasally with influenza virus (H1N1). Virus titres in the lung of mice administered either live or non-live L-92 cells daily for 15 d were repressed 6 d after virus infection compared with the control group. Natural killer (NK) activity in the orally administered non-live L-92 group was higher compared with that of the control group before virus infection and on day 6. In contrast, NK activity in the live L-92 group compared with the control group was not significantly changed on both days, but was significantly higher on day 1. In contrast, live L-92 showed a greater repression of virus proliferation compared with non-live L-92, 6 d after the infection. Live L-92 decreased the number of neutrophils in the lung and suppressed lung weight, leading to the consequent deterioration of consolidation scores of the lung. These results indicated that pretreatment of live or non-live L-92 cells had protective effects against influenza virus infection. Among the measured cytokines and chemokines, eotaxin, macrophage colony-stimulating factor, IL-1 beta, RANTES (regulated on activation, normal T cell expressed and secreted) and interferon-a were significantly increased in the lung: IL-17 was significantly increased in Peyer's patch of the live L-92 group compared with the control group. A mechanistic study suggested that the enhancement of NK activity in the lung caused by stimulating various antiviral cytokines and chemokines after the oral administration of L-92 cells might be important in protecting against virus infection.

引用

页码：1810 / 1818

页数：9

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