Complex polysaccharides as PCR inhibitors in feces: Helicobacter pylori model

被引：401

作者：

Monteiro, L

Bonnemaison, D

Vekris, A

Petry, KG

Bonnet, J

Vidal, R

Cabrita, J

Megraud, F

机构：

[1] UNIV BORDEAUX 2, BACTERIOL LAB, F-33076 BORDEAUX, FRANCE

[2] UNIV BORDEAUX 2, LAB IMMUNOL MOL, F-33076 BORDEAUX, FRANCE

[3] UNIV BORDEAUX 2, LAB BIOCHIM & BIOL CELLULAIRE, F-33076 BORDEAUX, FRANCE

[4] INSERM, BORDEAUX, FRANCE

[5] FAC FARM LISBON, LISBON, PORTUGAL

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1997年 / 35卷 / 04期

关键词：

D O I：

10.1128/JCM.35.4.995-998.1997

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A model was developed to study inhibitors present in feces which prevent the use of PCR for the detection of Helicobacter pylori. A DNA fragment amplified with the same primers as H. pylori was used to spike samples before extraction by a modified QIAamp tissue method. Inhibitors, separated on an Ultrogel AcA44 column, were characterized. Inhibitors in feces are complex polysaccharides possibly originating from vegetable material in the diet.

引用

页码：995 / 998

页数：4

共 25 条

[1] POLYMERASE CHAIN-REACTION FOR DIAGNOSIS OF ENTEROHEMORRHAGIC ESCHERICHIA-COLI INFECTION AND HEMOLYTIC-UREMIC SYNDROME [J].