CD200 Flow Cytometric Assessment and Semiquantitative lmmunohistochemical Staining Distinguishes Hairy Cell Leukemia From Hairy Cell Leukemia-Variant and Other B-Cell Lymphoproliferative Disorders

被引：32
作者：
Pillai, Vinodh
Pozdnyakova, Olga
Charest, Karry
Li, Betty
Shahsafaei, Aliakbar
Dorfman, David M. [1 ]
机构：
[1] Brigham & Womens Hosp, Dept Pathol, Boston, MA 02115 USA
来源：
AMERICAN JOURNAL OF CLINICAL PATHOLOGY | 2013年 / 140卷 / 04期
关键词：
Chronic lymphocytic leukemia; Mantle cell lymphoma; Hairy cell leukemia; Hairy cell leukemia-variant; OX-2 MEMBRANE GLYCOPROTEIN; TRANSCRIPTION FACTOR; THERAPEUTIC TARGET; PROGNOSTIC-FACTOR; T-BET; EXPRESSION; MALIGNANCIES; SUBSET; PD-1;
D O I：
10.1309/AJCPEBK31VQQNDDR
中图分类号：
R36 [病理学];
学科分类号：
100104 ;
摘要：
Objectives: To evaluate CD200 expression in B-cell proliferative disorders. Methods: We analyzed 180 recent specimens of B-cell neoplasms for CD200 expression by flow cytometric immunophenotypic analysis, which is better able to assess relative intensity of staining than immunohistochemical staining. Results: We found that hairy cell leukemia exhibits a high level of staining for CD200 in comparison to other B-cell lymphoproliferative disorders, including hairy cell leukemia-variant (HCL-V), marginal zone lymphoma, and lymphoplasmacytic lymphoma. We confirmed this observation by semiquantitative immunohistochemical staining. Conclusions: Assessment of the CD200 expression level is helpful to distinguish HCL from HCL-V and other B-cell lymphoproliferative disorders and in the differential diagnosis of B-cell neoplasms in general.
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收藏
页码：536 / 543
页数：8
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